Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session T44: Optical methods in therapy and imagingFocus
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Sponsoring Units: GMED DBIO Chair: Robert H. Austin; Matija Milanic, University of Ljubljana Room: Room 316 |
Thursday, March 9, 2023 11:30AM - 11:42AM |
T44.00001: Microstructure-Dependent Optical Properties of Doped Spinel Oxide Nanosystems David Beke, Adam Gali, Mátyás M Rudolf, Bence G Markus |
Thursday, March 9, 2023 11:42AM - 11:54AM |
T44.00002: Non-opsin-based biological vision: the physics of infrared light sensing in the pit viper Zachary Miller, Abby Leung, Trevor B Arp, Nathaniel M Gabor A unique set of sensory organs in certain snakes allow for sub 100 ms reaction with 5 degree accuracy in low light environments without the use of opsin based conformation-change photon detection that is nearly ubiquitous in natural systems. The capabilities of these organs center around a thin membrane sparsely populated with thermally sensitive 'pixels' capable of detecting mK temperature differences. Each membrane is situated in a shallow cavity or 'pit', the entrance of which is smaller than the area of the heat detecting membrane thus creating a rough pinhole camera. While much has been learned about the biochemistry of these sensory organs, questions of the mechanism of photon absorption, the reasons behind their geometry, how accurate imaging is obtained from a noisy environment, and how visual information is reconstructed all remain unanswered. To address these, we employ an experimental realization of a pit membrane using an InGaAs detector translated within a model pit. While exploring different optical geometries, we reconstruct thermal images using a geometrically inspired single step algorithm with noise parameters related to input and detector noise. Understanding infrared sensing in pit vipers promises new insight into the physics and biology of light sensing in natural systems, beyond the more common opsin based mechanisms. |
Thursday, March 9, 2023 11:54AM - 12:06PM |
T44.00003: Modular Hyperspectral Imaging System Matija Milanic, Jost Stergar, Luka Rogelj, Tadej Tomanic Hyperspectral imaging (HSI) combines spectroscopy with imaging, providing large field-of-view (FOV) and sensitivity to both molecular and morphological properties of samples. Recently multiple different clinical and preclinical applications of HSI have been reported. The commercially available HSI systems lack modularity and adaptability, reducing their applicability in clinical and preclinical environment. |
Thursday, March 9, 2023 12:06PM - 12:42PM |
T44.00004: State-of-the-Art Technologies for Optical Imaging of Biological Tissues: Contrast, Resolution, and Throughput Invited Speaker: Yuankai Tao Optical techniques and instrumentation play a significant role in biomedical science and medicine. State-of-the-art biomedical research requires a high level of sophistication in contemporary optical technologies, including fluorescence contrast, advanced microscopy, volumetric imaging. This talk will provide an overview of applications of optical imaging in biological tissues including methods such as fluorescence and nonlinear contrast, super-resolution, and holography and interferometry. These technologies will be presented in the context of overcoming fundamental limitations of optical imaging to enable in vivo visualization of cellular and subcellular features and interactions; surpassing the diffraction-limit using novel fluorophores and computational methods; and pushing the limits of data acquisition speeds for high spatial/temporal imaging throughput. |
Thursday, March 9, 2023 12:42PM - 12:54PM |
T44.00005: Blue-light optical coherence microscopsy Shanjida Khan, Kai Neuhaus, Omkar Thaware, Shuibin Ni, Myeong J Ju, Travis Redd, David Huang, Yifan Jian Corneal disease is the fifth leading cause of visual loss globally. The cornea, the optically transparent and outermost part of the eye, is vital for light transmission. Currently, specular microscopy (SM) and in vivo confocal microscopy (IVCM) technologies are used in clinical settings for cell evaluation, offering very high lateral resolution to observe individual cells and even cell nuclei in corneal tissue. However, SM and IVCM have some disadvantages, such as limited field of view, resulting in prolonged image acquisition and processing time, and lack of volumetric imaging capability. Optical coherence tomography (OCT), which is a non-invasive imaging modality, systems mostly use low numerical aperture (NA) objectives combined with broadband sources such as supercontinuum, femtosecond lasers, and a less expensive alternative, super-luminescent diode, to achieve high axial resolution, but the low NA objectives ultimately limit the lateral resolution to tens of microns. Optical coherence microscopy (OCM) is an imaging modality based on OCT and confocal microscopy that incorporates large NA objectives to achieve high lateral resolution. In this study, we present a blue-light OCM system with a center wavelength of 450 nm and a 6 nm bandwidth. We demonstrated high lateral resolution images of ex vivo rabbit corneal cellular structures such as epithelial cells, endothelial cells, keratocytes, and stromal collagen lamellae. |
Thursday, March 9, 2023 12:54PM - 1:06PM |
T44.00006: Accurate Henle’s Fiber Layer Measurement by Volumetric Directional OCT Shuibin Ni, Shanjida Khan, Thanh-Tin P. Nguyen, Ringo Ng, Brandon Lujan, Ou Tan, David Huang, Yifan Jian Progressive photoreceptor cell body loss could result in the thinning of the outer nuclear layer (ONL), which is an important pathological feature of retinal degenerations and a useful imaging biomarker in age-related macular degeneration. However, it is challenging to demarcate the boundary between the ONL and the adjacent Henle's fiber layer (HFL), which consists of Müller cell and obliquely oriented photoreceptor axons, by standard optical coherence tomography (OCT). We proposed a novel imaging technology, Volumetric Directional OCT (VD-OCT), a dedicated OCT system with two sets of optical scanners that can precisely control and continuously and synchronously update the imaging beam entry points during a volumetric OCT scan. A customized spoke-circular scanning pattern was also implemented to observe the appearance of HFL with sufficient optical contrast in continuous cross-sectional scans through the entire volume. The application of VD-OCT for retinal imaging provides a more reliable and efficient approach to clearly distinguish the structure between the ONL and HFL. The novel imaging technology has the potential to allow for early identification of retinal diseases. |
Thursday, March 9, 2023 1:06PM - 1:18PM |
T44.00007: Larrea tridentata: A path to a non-invasive brain cancer treatment Lizbeth V Martinez Lopez, Felicia S Manciu, Kevin E Bennet, Marian Manciu Astrocytes, one of the most abundant cell components in the central nervous system (CNS), have been a research target in the last few years. Several studies have found that astrocytes are not only mere supporters of neurons but also of essential processes developed in the CNS. Their malfunction could induce neurodegenerative diseases and brain tumors. Thus, further understanding of astrocytes and their role is of high interest to develop possible new treatments and methods of disease diagnosis, especially in brain cancer. The plant Larrea tridentata (La Gobernadora in Mexico or Creosote bush in the United States) is known to have medical properties for different disorders, including cancer, due to one of its components, the nordihydroguaiaretic acid (NDGA). By using confocal Raman microscopy to analyze the function of NDGA in normal astrocytes and glioblastoma, we found evidence that this acid does not affect the former, making this a potential non-invasive alternative for cancer treatment. |
Thursday, March 9, 2023 1:18PM - 1:30PM |
T44.00008: Quantification of Malaria Parasite in Blood Smears using Phase Microscopy Charlotte Kyeremah, David Newey, Albert A Adu, Wisdom S Mensah, Daniel Haehn, Chandra S Yelleswarapu Early and accurate diagnosis of malaria in the human red blood cells is acute to the success of eliminating malaria. The method of malaria diagnosis using optical microscopy involves staining and manually counting infected cells by trained personnel, making the process subjective, time-consuming, and labor-intensive. We present the results of phase microscopy using an automated digital processing technique for malaria parasite detection and identification of species and stages. |
Thursday, March 9, 2023 1:30PM - 1:42PM |
T44.00009: The effects of hypoxia on cancer cell motility and on cell size Robert H Austin, Kimberly Shen, Noreen Hosny, Kenneth J Pienta, Sarah Amend, Emma Hammarlund We investigate the effects of hypoxia on cancer cell motility (as a potential indicator of EMT) and on cell size (as a potential indicator of PACC formation). In particular, we subject cancer cells to self-generated hypoxia, that is, hypoxic conditions resulting from the cancer cells' own metabolism, closely resembling the process that occurs naturally in tumors. Previous studies have attempted to create hypoxic conditions by either purging the cell environment of oxygen or by using cobalt chloride. However, the former method suffers from an inability to create oxygen gradients which are crucially present in tumors and the latter method is merely mimetic: it induces a hypoxia-like response in cells by stabilizing certain protein factors but does not physically decrease ambient oxygen levels. Lastly, both methods are externally imposed rather than emergent from the tumor itself, giving uncertainty to the applicability of the observed cell responses to the behavior of cells in real tumors. |
Thursday, March 9, 2023 1:42PM - 1:54PM |
T44.00010: Fourier space analysis of tissue order Jost Stergar, Katja Lakota, Martina Perše, Matija Tomši?, Nika Kojc, Matija Milanic Tissue architecture is one of the paramount aspects of diagnostic pathology and is, as such, of great importance in development of digital pathology. Inflammation can result in tissue damage, changing the tissue organization. To evaluate the disease progression and severity, the extent and intensity of these changes must be determined. |
Thursday, March 9, 2023 1:54PM - 2:06PM |
T44.00011: Hyperspectral imaging and optical profilometry of murine tumor models Tadej Tomanic, Luka Rogelj, Jost Stergar, Bostjan Markelc, Tim Bozic, Simona Kranjc Brezar, Gregor Sersa, Matija Milanic Cancer is one of the leading causes of death worldwide. There is a pressing need to diagnose cancer early and monitor it as the disease progresses or recedes. Tissue physiology and morphology are significantly different in tumors compared to healthy tissues, and these changes could be detected noninvasively using optical imaging techniques. |
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