Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session T44: Optical methods in therapy and imaging
11:30 AM–2:06 PM,
Thursday, March 9, 2023
Room: Room 316
Sponsoring
Units:
GMED DBIO
Chair: Robert H. Austin; Matija Milanic, University of Ljubljana
Abstract: T44.00010 : Fourier space analysis of tissue order*
1:42 PM–1:54 PM
Presenter:
Jost Stergar
(Jozef Stefan Institute)
Authors:
Jost Stergar
(Jozef Stefan Institute)
Katja Lakota
(University Medical Centre, Department of Rheumatology, Vodnikova ulica 62, 1000 Ljubljana, Slovenia)
Martina Perše
(Faculty of Medicine,University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia)
Matija Tomši?
(University Medical Centre, Department of Rheumatology, Vodnikova ulica 62, 1000 Ljubljana, Slovenia)
Nika Kojc
(Faculty of Medicine,University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia)
Matija Milanic
(University of Ljubljana)
We present an approach to determine the overall order of tissue architecture in histology slides of murine abdominal wall samples with induced peritonitis. Hyperspectral microscopy between 450 nm and 750 nm is used to acquire an image of a standard H&E slide. Using the law of exponential attenuation, the image is decomposed into maps of both stains. A Fourier transformation is applied to the eosin image of cells, obtaining a distribution of spatial frequencies at different directions in the tissue. Where tissue architecture was impaired, the distribution was independent of the angle, whereas in healthy, well-organized tissues, a dominant spatial frequency direction was observed. The method was used for classification of subjects into healthy and diseased, achieving a p-value of 0.02.
The presented approach could serve as a basis for future development of quantitative metrices in digital pathology, aiding in automated, computer-assisted assessment, facilitating larger screening and longitudinal studies.
*Funding: ARRS P1-0389 and Z1-4384
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