Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session T44: Optical methods in therapy and imaging
11:30 AM–2:06 PM,
Thursday, March 9, 2023
Room: Room 316
Sponsoring
Units:
GMED DBIO
Chair: Robert H. Austin; Matija Milanic, University of Ljubljana
Abstract: T44.00011 : Hyperspectral imaging and optical profilometry of murine tumor models*
1:54 PM–2:06 PM
Presenter:
Tadej Tomanic
(University of Ljubljana)
Authors:
Tadej Tomanic
(University of Ljubljana)
Luka Rogelj
(Univ of Ljubljana)
Jost Stergar
(Jozef Stefan Institute)
Bostjan Markelc
(Institute of Oncology Ljubljana)
Tim Bozic
(Institute of Oncology Ljubljana)
Simona Kranjc Brezar
(Institute of Oncology Ljubljana)
Gregor Sersa
(Institute of Oncology Ljubljana)
Matija Milanic
(University of Ljubljana)
In this study, we combined hyperspectral imaging (HSI) with 3D optical profilometry (OP) to image multiple subcutaneously grown murine tumor models. Tissue properties such as melanin volume fraction and blood oxygenation were extracted from HSI data using the inverse adding-doubling (IAD) algorithm. Blood vessels were segmented from tissue oxygenation maps using the B-COSFIRE algorithm, and tumor volumes were calculated from 3D OP data and compared to manual measurements by a caliper.
We confirm that tumors can be discriminated from healthy tissue based on the differences in the measured reflectance spectra and extracted tissue parameters (p<0.05). The results also indicate that blood oxygenation is 50% higher in the blood vessels than in the surrounding tissue. Finally, we show that the tumor volumes estimated using OP deviate up to 26% from the manual measurements. Our study demonstrates that the combined HSI and OP imaging can successfully monitor the evolution of tumors in murine models.
*This study was supported by the Slovenian Research Agency (ARRS) (grant numbers P1-0389, P3-0003, J3-2529, and J3-3083).
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