Bulletin of the American Physical Society
75th Annual Gaseous Electronics Conference
Monday–Friday, October 3–7, 2022;
Sendai International Center, Sendai, Japan
The session times in this program are intended for Japan Standard Time zone in Tokyo, Japan (GMT+9)
Session HT4: Poster Session I (4:00-6:00pm, JST)
Tuesday, October 4, 2022
Sendai International Center Room: Sakura 1
Abstract: HT4.00096 : Mechanism of macromolecular introduce into plant cells by plasma treatment.*
(Pearl Kogyo Co.,Ltd)
The callus was placed statically in a 3.5 cm dish on a GND electrode. A needle electrode was placed above the callus, and plasma was irradiated directly to the cells by applying a sinusoidal voltage. Twenty-four hours after plasma treatment, the callus was stained with X-Gluc to observe the expression of GUS. GUS expression was observed on the cell treated two times, though no GUS expression was observed for single-time treatment. After the plasma treatment, the cell surface of the callus was observed by FE-SEM. The callus treated two times scraped the cell wall and other parts of the introduction barrier more deeply than the callus treated only one time.
These results suggest that by multi-time plasma treatment, the target macromolecules, such as GUS plasmid, reach the cell membrane through the cracks in the cell wall, then they are introduced into cells by endocytosis mediated by the complex stimuli of plasma.
*This work was supported by JSPS KAKENHI Grant Number 21H04455, 17H01068.
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