Bulletin of the American Physical Society
75th Annual Gaseous Electronics Conference
Volume 67, Number 9
Monday–Friday, October 3–7, 2022;
Sendai International Center, Sendai, Japan
The session times in this program are intended for Japan Standard Time zone in Tokyo, Japan (GMT+9)
Session EF4: Plasma Medical & Agricultural Application IV
4:00 PM–5:30 PM,
Friday, October 7, 2022
Sendai International Center
Room: Hagi
Chair: Kenji Ishikawa, Nagoya University, Japan
Abstract: EF4.00004 : Analysis of cell exposed to non-thermal atmospheric pressure plasma for effective gene transfer*
5:00 PM–5:15 PM
Presenter:
Shinya Kumagai
(Meijo University)
Authors:
Tatsuya Kitazaki
(Meijo University)
Linhao Sun
(Kanazawa University)
Han N GIA
(Kanazawa Univesity)
Shinji Watanabe
(Kanazawa University)
Shinya Kumagai
(Meijo University)
Murine fibroblast cells (L929) were exposed to NTAPP using a plasma jet source (He 2 slm; 9 kV; 12. 5 kHz) and fluorescent reagent (DiYO-1) was supplied to the cells. After 1 h incubation, the cells were observed by fluorescent microscopy.
NTAPP exposure more than 13 s introduced DiYO-1 into the cells. Then, glutaraldehyde was supplied to the cells for fixing their surface morphology. The cells were freeze-dried for scanning electron microscopy. For the cells exposed to NTAPP, pores were found on the cell membrane.
To exclude the possibility that the pores were formed during freeze-drying, the cells were observed in liquid medium using scanning ion microscopy (SICM) [2]. SICM revealed pores on the cell membrane. The pores formed by NTAPP exposure can play important roles in gene delivery.
[1] S. Kumagai et al., Jpn. J. Apple. Phys., 55, 01AF01 (2016) (OPEN ACCESS); https://doi.org/10.7567/JJAP.55.01AF01
[2] S. Watanabe et al., Rev. Sci. Instrum. 90, 123704 (2019); https://doi.org/10.1063/1.5118360
*This work was supported by JSPS KAKENHI Grant No. 18K19942 and 19H04457, The Uehara Memorial Foundation and The Hibi Science Foundation.
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