Bulletin of the American Physical Society
APS March Meeting 2021
Volume 66, Number 1
Monday–Friday, March 15–19, 2021; Virtual; Time Zone: Central Daylight Time, USA
Session H71: Poster Session I (2:00pm - 4:00pm)
2:00 PM,
Tuesday, March 16, 2021
Abstract: H71.00005 : Aggregation of HEK-293 Tumorigenic Cell Line Using Sound Waves
Presenter:
Karen Ngo
(California State University, San Marcos)
Authors:
Karen Ngo
(California State University, San Marcos)
Banaz Qasab
(California State University, San Marcos)
Carlos Luna
(California State University, San Marcos)
We utilized 3D printing to prototype acoustic chambers and piezoelectric material to generate sound waves. HEK-293 tumorigenic cell line and HeLa cells were subjected to 523.25 Hz to 987.77 Hz, from 0-20V, for 30 min to 24 hrs at 37C. Cells were imagined using live-microscopy and aggregates were stained for nuclei (DAPI) and actin (phalloidin).
Rate of cell movement was inversely proportional to frequency and directly proportional to amplitude. The aggregation of cells was minimized by cell attachment to the dish. For future experiments, newly fabricated sound chambers can be used to test and regulate the directionality of echoes produced by the sound waves.
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