Flow Analysis and UniverScope: complementary techniques to monitor spheroid growth*

The Bioimaging & Optofluidics laboratory has developed an encapsulation technique that allows to produce multicellular spheroids as model tissue at a very high throughput and controlled manner.¹ To monitor the growth of spheroids, we use two complementary techniques. The first one relies on an image-free analysis of the light absorbed by the spheroids; the second one consists of building a microscope that allows parallelized imaging of spheroids in physiological conditions. These two methods are aimed at measuring the spheroid radius as a function of time and at gaining insight into the fate of cells within the spheroids.


^{1. Kevin Alessandri,, Laetitia Andrique, Maxime Feyeux, Andreas Bikfalvi, Pierre Nassoy & Gaëlle Recher. “All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide”. Scientific Reports. Volume Article number: 42378 (2017).}