Bulletin of the American Physical Society
APS March Meeting 2019
Volume 64, Number 2
Monday–Friday, March 4–8, 2019; Boston, Massachusetts
Session V65: Membranes and Channels
2:30 PM–5:30 PM,
Thursday, March 7, 2019
BCEC Room: 260
Sponsoring Unit: DBIO
Chair: Arman Fathizadeh, University of Texas at Austin
Abstract: V65.00014 : Improving membrane protein expression by optimizing simulated integration efficiency
5:06 PM–5:18 PM
Co-translational insertion into the cell membrane via the Sec translocon is a key step in the production of IMPs. However, both experimental and computational studies of this process are difficult due to the role of long-timescale non-equilibrium dynamics during ribosomal translation. We have developed a coarse-grained molecular dynamics (CGMD) approach that is capable of reaching experimentally relevant (i.e. minute) timescales, while retaining the level of detail required to capture the effect of individual amino-acid substitutions.
Using a combination of simulations of IMP integration and experimental quantification of IMP expression levels; we demonstrate a link between the efficiency of IMP integration into the cell membrane and IMP expression, yielding a powerful tool for the rational prediction of sequence modifications that improve expression.
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