Anti-cancer drug containing apolioprotein B lipoparticle reconstitution and tracking in living cell*

Radicicol (Rad), an Hsp 90 inhibitor, is an antitumor drug which loses its antitumor activity during delivery process, in vivo. By adding Rad in the reconstituting apolioprotein B (apoB) process, a Rad containing reconstituted apoB lipoparticle (rABL) complex (Rad@rABL) can be reconstituted and characterized by photonic spectroscopies, differential scanning calorimetry and super resolution imaging. Interestingly, the Rad@rABL single molecule can be tracked within the living cell. The drug delivery route to the mitochondria in the Hep G2 cell line can be monitored. Moreover, the anticancer efficacy of Rad can be enhanced. In summary, rABL is an excellent and native self-assembly drugs carrier and living cell tracking agent.