Bulletin of the American Physical Society
APS March Meeting 2019
Volume 64, Number 2
Monday–Friday, March 4–8, 2019; Boston, Massachusetts
Session K59: Rheology of Active Fluids: From Active Polymers to Living Matter
8:00 AM–11:00 AM,
Wednesday, March 6, 2019
BCEC Room: 257B
Sponsoring Unit: GSOFT
Chair: Wylie Ahmed, California State University, Fullerton
Abstract: K59.00003 : Spontaneous migration of cellular aggregates: from giant keratocytes to running spheroids
8:48 AM–9:00 AM
Here, we investigate the collective migration of cell on adhesive gels, using 3D cellular aggregates as a model system. Aggregates spread by expanding outwards a cell monolayer, which may partially dewet, causing the aggregate to move. Varying the substrate rigidity induces different modes of aggregate motion: “Giant Keratocytes”, where the lamellipodium is a cell monolayer that expands at the front and retracts at the back; “Penguins”, characterized by bipedal locomotion; and “Running Spheroids”, for non-spreading aggregates. We characterize these diverse modes of collective migration by quantifying the flows and force field responsible of the bipedal stick-slip motion.
We propose two possible mechanisms to explain the spontaneous migration of cellular aggregates: i)chemical modification of the substrate in analogy to reactive droplets. We show that it is possible to mimic a keratocyte with a droplet of oil containing a surfactant. The reactive droplet adopts a croissant shape also seen for keratocyte fragments and ii) symmetry-breaking arising from cell polarization in analogy to active droplets.
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