Bulletin of the American Physical Society
APS March Meeting 2019
Volume 64, Number 2
Monday–Friday, March 4–8, 2019; Boston, Massachusetts
Session A64: Physics in Synthetic Biology
8:00 AM–11:00 AM,
Monday, March 4, 2019
BCEC Room: 259B
Sponsoring Unit: DBIO
Chair: Gabor Balazsi, State Univ of NY - Stony Brook
Abstract: A64.00004 : On the noise of gene expression in membrane-bound picolitre biochemical reactors
9:24 AM–9:36 AM
(Physics and Nanotechnology, University of Minneapolis)
Yusuke T. Maeda
In this presentation will be shown a new microfluidic setup that allows the screening of gene expression in a large number (N>1000) of identical picolitre microwells sealed by a biological membrane.
Gene expression of GFP using a cell-free transcription-translation system is performed in picolitre microreactors sealed by a membrane made of a mixture of phospholipids. Each experiment stands for a model clonal population. Although the kinetics of gene expression follows the same master curve for all the microwells, the end-points of the gene expression revealed a variability in the amount of protein produced, with a long right-tail distribution. This observation holds regardless of the composition of the outer phase, emphasizing this intrinsic property of the artificial cells.
 H. Salman, N. Brenner, C.-K. Tung, N. Elyahu, E. Stolovicki, L. Moore, A. Libchaber and E. Braun, Universal Protein Fluctuations in Populations of Microorganisms, Phys. Rev. Lett. 108, 238105 (2012)
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