2009 APS March Meeting
Volume 54, Number 1
Monday–Friday, March 16–20, 2009;
Pittsburgh, Pennsylvania
Session H24: Focus Session: Chemical Modification of Nanotubes
8:00 AM–11:00 AM,
Tuesday, March 17, 2009
Room: 326
Sponsoring
Unit:
DCMP
Chair: Traian Dumitrica, University of Minnesota
Abstract ID: BAPS.2009.MAR.H24.1
Abstract: H24.00001 : Single Molecule Detection in Living Biological Cells using Carbon Nanotube Optical Probes
8:00 AM–8:36 AM
Preview Abstract
Abstract
Author:
Michael Strano
(MIT)
Nanoscale sensing elements offer promise for single molecule
analyte detection in physically or biologically constrained
environments. Molecular adsorption can be amplified via
modulation of sharp singularities in the electronic density of
states that arise from 1D quantum confinement [1]. Single-walled
carbon nanotubes (SWNT), as single molecule optical sensors
[2-3], offer unique advantages such as photostable near-infrared
(n-IR) emission for prolonged detection through biological media,
single-molecule sensitivity and, nearly orthogonal optical modes
for signal transduction that can be used to identify distinct
classes of analytes. Selective binding to the SWNT surface is
difficult to engineer [4]. In this lecture, we will briefly
review the immerging field of fluorescent diagnostics using band
gap emission from SWNT. In recent work, we demonstrate that even
a single pair of SWNT provides at least four optical modes that
can be modulated to uniquely
fingerprint chemical agents by the degree to which they alter
either the emission band intensity or wavelength. We validate
this identification method in vitro by demonstrating detection
and identification of six genotoxic analytes, including
chemotherapeutic drugs and reactive oxygen species (ROS), which
are spectroscopically differentiated into four distinct
classes. We also demonstrate single-molecule sensitivity in
detecting hydrogen peroxide, one of the most common genotoxins
and an important cellular signal. Finally, we employ our sensing
and fingerprinting method of these analytes in real time within
live 3T3 cells, demonstrating the first multiplexed optical
detection from a nanoscale biosensor and the first
label-free tool to optically discriminate between genotoxins. We
will also discuss our recent efforts to fabricate biomedical
sensors for real time detection of glucose and other important
physiologically relevant analytes in-vivo. The response of
embedded SWNT in a swellable hydrogel construct to
osmotic pressure gradients will be discussed, as well as its
potential as a unique transduction mechanism for a new class of
implantable sensors.
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[1] Saito, R., Dresselhaus, G. {\&} Dresselhaus, M. S.
\textit{Physical Properties of Carbon Nanotubes }(Imperial
College Press, London, 1998).
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[2] Barone, P. W., Baik, S., Heller, D. A. {\&} Strano, M. S.
Near-Infrared Optical Sensors Based on Single-Walled Carbon
Nanotubes. \textit{Nature Materials }\textbf{4}, 86-92 (2005).
\\[0pt]
[3] Jeng, E. S., Moll, A. E., Roy, A. C., Gastala, J. B. {\&}
Strano, M. S. Detection of DNA hybridization using the near
infrared band-gap fluorescence of single-walled carbon nanotubes.
\textit{Nano Letters }\textbf{6},
371-375 (2006).
\\[0pt]
[4] Heller, D. A. et al. Optical detection of DNA conformational
polymorphism on single-walled carbon nanotubes.
\textit{Science }\textbf{311}, 508-511 (2006).
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2009.MAR.H24.1