Extraction of complementary from non-complementary DNA sequences through phase separation and centrifugation

11:15 AM–2:15 PM, Thursday, March 13, 2008
Morial Convention Center Room: 232

Sponsoring Unit: DBP
Chair: Noel Clark, University of Colorado

Abstract ID: BAPS.2008.MAR.V40.7

Abstract: V40.00007 : Extraction of complementary from non-complementary DNA sequences through phase separation and centrifugation*

12:27 PM–12:39 PM

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Authors:

Taiquitha Robins
(Jackson State University, Jackson, MS)

Dacia McPherson
(Department of Physics, University of Colorado, Boulder, CO)

Chenhui Zhu
(Department of Physics, University of Colorado, Boulder, CO)

Mark Moran
(Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO)

Dave Walba
(Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO)

Giuliano Zanchetta
(Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universit\`a di Milano, Milano, Italy)

Tommaso Bellini
(Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Universit\`a di Milano, Milano, Italy)

Noel Clark
(Department of Physics and Liquid Crystal Materials Research Center, University of Colorado, Boulder, CO)

Double stranded deoxyribonucleic acid (DNA) is known to form lyotropic liquid crystal (LC) phases, nematic and then columnar with increasing DNA concentration in water. Single stranded (DNA) does not form liquid crystal phases. We study the phase separation of both long (900bp) and short (6-20bp) DNA. In the mixture solution of a self complementary sequences (scDNA) and non complementary sequences (nscDNA), the scDNA forms DNA double helices and hence forms LC phases while the nscDNA stays in the isotropic phase, the LC appearing in the form of phase separated droplets. We report results of the use of centrifugation to produce complete spatial segregation of complementary and noncomplementary DNA, based on their different LC-formation tendencies.

*This work is supported by NSF MRSEC Grant DMR 0213918 and NSF Grant ITS-0606528.

To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2008.MAR.V40.7

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Bulletin of the American Physical Society

2008 APS March Meeting

Volume 53, Number 2

Monday–Friday, March 10–14, 2008; New Orleans, Louisiana

Session V40: Nucleic Acids: Structure and Function

Abstract: V40.00007 : Extraction of complementary from non-complementary DNA sequences through phase separation and centrifugation*

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