Investigation on Reaction of Plasma-generated Dinitrogen Pentoxide Gas with Amino Acids*

Atmospheric-pressure plasma (APP) technology, enabling to convert air molecules into reactive species [e.g., reactive oxygen and nitrogen species (ROS and RNS)] with electricity, is of great interest and has been extensively investigated. Recently, we have developed a new air APP device/method that allows highly selective production of dinitrogen pentoxide (N₂O₅) exclusively from only air and electricity sources. In addition, we demonstrated the plasma-generated N₂O₅ gas elicited activation of plant immunity and plant disease resistance was enhanced in Arabidopsis thalianaexposed to the N₂O₅ gas. In this way, N₂O₅ is a promising chemical for a variety of applications, while the fundamental experiments using N₂O₅ are still scarce. In this study, we focused on the interaction of the plasma-generated N₂O₅ gas with several standard amino acids such as tyrosine and cysteine, and have investigated plasma-generated reactive species and amino-acid derivatives. In particular, composition of tyrosine derivatives generated by the treatment of the plasma-generated N₂O₅ gas strongly depended on reactive species other than N₂O₅ transferred into solution. In the presentation, we will introduce results using some kinds of amino acids derivatives and discuss the reaction mechanisms.