Bulletin of the American Physical Society
75th Annual Gaseous Electronics Conference
Volume 67, Number 9
Monday–Friday, October 3–7, 2022;
Sendai International Center, Sendai, Japan
The session times in this program are intended for Japan Standard Time zone in Tokyo, Japan (GMT+9)
Session EF3: Plasma Medical & Agricultural Application III |
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Chair: Jun-Seok Oh, Osaka Metropolitan University Room: Sendai International Center Hagi |
Friday, October 7, 2022 1:30PM - 2:00PM |
EF3.00001: Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells Invited Speaker: Nevena Puac The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions. |
Friday, October 7, 2022 2:00PM - 2:15PM |
EF3.00002: Reproducibility in plasma agriculture Masaharu Shiratani, Teruki Anan, Takumi Nakao, Takamasa Okumura, Pankaj Attri, Kazunori Koga In a survey of 1,576 scientists published in Nature,1) 90% acknowledged reproducibility crisis. The survey reported that over 70% scientists failed to reproduce others' experiments and over 50% failed to reproduce their own results. Experimental results fail reproducibility tests due to several reasons including improper documentation of methodology, considering noise as a positive finding, incomplete experimental variables, data fabrication or bias, publishing incomplete results and inappropriate statistical analysis.2) In plasma agriculture, reproducibility depends on living things such as seeds, cultivation environment, particle fluxes from plasma, and interactions between them. Based on experimental results, we identified some important factors such as seed coat color and humidity in air to realize good reproducibility.3) Information on such factors should be described in published papers as well as laboratory notebooks. |
Friday, October 7, 2022 2:15PM - 2:30PM |
EF3.00003: Various approaches of cold plasma treatment to brewer's rice plant for improvement of grain quality Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Kaoru Sanda, Genki Yuasa, Satoe Tohno, Shih-Nan Hsiao, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Yoji Hirosue, Masayoshi Maeshima, Masaaki Mizuno, Masaru Hori The applications of cold plasma in agriculture have much attention. We have investigated the efficacy of cold plasma treatment in the field for rice cultivation. We focused on the rice cultivation process of brewer’s cultivar in this study, and treated not only the seedlings in a paddy but also the spikelets, using rice plants cultivated in the University paddy field in Togo town, Aichi, with cold plasma; direct plasma irradiation and indirect treatment with plasma-activated Ringer’s lactate solution. As a result of evaluating the quality of brown rice after harvest, in any ways, the ratio of white-core grains, which is the important structure for brewing Japanese wine (sake), to the whole ones was increased under the optimal condition. These results indicated that the various approaches of plasma treatment according to the rice cultivation can improve the quality of brown rice. |
Friday, October 7, 2022 2:30PM - 2:45PM |
EF3.00004: Surface Modification Analysis of the Closed Containers that are used in Plasma Treatments of Food, Agriculture, and Biomedical Samples Naman Bhatt, Joshua Morsell, Duncan P Trosan, Patrick D Walther, Katharina Stapelmann, Steven Shannon Plasma treatments inside closed containers is ideal for food, agriculture, and biomedical samples to mitigate post-process contamination. Typical containers are plastic bags that are made out of low-density polyethylene (LDPE), polyethylene terephthalate (PET), nylon, and Teflon FEP polymers. The transparent heat-sealable bags offer an easy solution for plasma treatment of delicate samples. However, the issue of polymer degradation and interference with the sample processing has to be addressed. An alternative plasma apparatus is discussed that allows plasma treatments inside sealed glass vials. |
Friday, October 7, 2022 2:45PM - 3:00PM |
EF3.00005: Effect of plasma irradiation on germination of lettuce seeds with fluctuating dormancy Teruki Anan, Takumi Nakao, Takamasa Okumura, Pankaj Attri, Kunihiro Kamataki, Naoto Yamashita, Naho Itagaki, Kazunori Koga, Masaharu Shiratani Induction of response of plant seeds by irradiation with non-thermal atmospheric pressure plasma has attracted attention [1-3]. Many studies have shown a phenotypic correlation to plasma irradiation conditions (voltage, power, gas, etc.). However, the elucidation of the mechanism has just begun. To elucidate the mechanism, improving the reproducibility of the plasma irradiation effect on seeds is essential. This study deals with the dormant state of seeds to be irradiated with plasma. The sample is lettuce seeds. The seeds were irradiated with plasma at different storage periods. Scalable dielectric barrier discharge electrodes were used for plasma irradiation [4]. After irradiation, the seeds were used for germination tests. The germination rate was counted every 12 hours after imbibition. As a result, the germination rate at 12 hours was 10% at 0d of storage, but 50% at 40d, and then gradually decreased to 10% at 150d. This result indicates a dormancy fluctuation depending on the storage period. Plasma-irradiated seeds show improvement in germination characteristics earlier than seeds without plasma irradiation. Thus, it is important to construct an experimental system considering the dormancy as an important parameter. The presentation will discuss the dependency of plasma irradiation time on germination of seeds with fluctuating dormancy. |
Friday, October 7, 2022 3:00PM - 3:15PM |
EF3.00006: Plasma irradiation-introduced RONS amount into plant seeds and their response analysis Takamasa Okumura, Teruki Anan, Pankaj Attri, Yuichi Tsukada, Kunihiro Kamataki, Naoto Yamashita, Naho Itagaki, Kazunori Koga, Masaharu Shiratani, Yushi Ishibashi Discharge plasma irradiates seeds with reactive oxygen and nitrogen species (RONS) and induces biological response [1-3]. However, RONS introduced in seeds by plasma irradiation have not been successfully detected thus far. This study provides experimental evidence that nitrate ion NO3- is introduced in lettuce seeds as RONS upon irradiation with atmospheric-pressure air dielectric barrier discharge plasma. Plasma irradiation for 5 min promotes seed germination. The components of the plasma-irradiated seeds were examined using electrospray ionization quantum mass spectrometry (ESI QMS), which revealed that the plasma irradiation introduced an ion with a mass of 62 m/z in detectable amounts. This ion was identified as NO3- by liquid chromatography (LC), multiple wavelength detector (MWD), and LC-ESI QMS. A one-dimensional simulation at electron temperature Te=1eV, electron density Ne=1013/m3, and gas temperature Tg=300 K indicated the introduction of NO3-, involving nitric oxide NO. NO3- is one of the most important ions that trigger signal transduction for germination when introduced in seeds. The scanning electron microscopy (SEM) images revealed that there was no change on the surface of the seeds after plasma irradiation. Plasma irradiation is an effective method of introducing NO3- in seeds in a dry process without causing damage. References [1] P. Attri, et al., Processes 2020, 8, 1002. [2] P. Attri, et al., Jpn. J. Appl. Phys., 2021, 20, 040502. [3] P. Attri, et al., Agronomy 2022, 12, 482. |
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