Bulletin of the American Physical Society
73rd Annual Gaseous Electronics Virtual Conference
Volume 65, Number 10
Monday–Friday, October 5–9, 2020; Time Zone: Central Daylight Time, USA.
Session KT4: Plasma Medicine and AgricultureLive
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Chair: Katharina Stapelmann, North Carolina State University |
Tuesday, October 6, 2020 3:00PM - 3:15PM Live |
KT4.00001: Role of seed coat color and harvest year on growth enhancement by plasma irradiation to seeds Kazunori Koga, Pankaj Attri, Kenji Ishikawa, Takamasa Okumura, Kayo Matsuo, Daisuke Yamashita, Kunihiro Kamataki, Naho Itagaki, Masaharu Shiratani, Vida Mildaziene In recent years non-thermal plasma (NTP) application in agriculture is rapidly increasing, although the response of seeds to NTP treatment may depend on the coat color and harvest year has never been tackled. Therefore, in this study we have used radish sprouts (Raphanus sativus L.) seeds treated with scalar DBD for 3 mins [1]. The seeds were bought from Nakahara Seed Co., Japan in 2017 and 2018. Seeds were separated into two color groups of brown and grey. It was observed that there was no significant difference in the germination rate of brown and grey seeds for both 2017 and 2018 harvest year after the NTP treatment. Whereas, growth enhancement was observed for 2017 harvest, while no significant growth enhancement was observed for 2018 harvest for both brown and grey color seed coat after NTP treatment. Later, we observed increased concentration of Gibberellin A3 after plasma treatment for 2017 harvest year, while there was no significant difference for 2018 harvest year. This shows that harvest year and seed coat color both plays significant role in growth enhancement after plasma treatment. However, the harvest year dominant over seed coat color in plasma agriculture. [1] K. Koga, et al., Jpn. J. Appl. Phys. 59 (2020) SHHF01. [Preview Abstract] |
Tuesday, October 6, 2020 3:15PM - 3:30PM Live |
KT4.00002: Low-temperature atmospheric pressure plasma accelerates quad seeds imbibition, germination, and speed of the seedling growth Alexander Volkov Low-temperature atmospheric pressure plasma can play an important role in agriculture, medicine and biophysical applications. Generated by the cold He-plasma jet reactive oxygen and nitrogen species, UV-Vis photons, and high-frequency strong electromagnetic fields with amplitude of a few kV can interact with seeds and plants. Here we show that cold plasma jet accelerates seed imbibition, germination, radicle and plants growing rates. Low-temperature atmospheric pressure helium plasma jet induces roughness, corrugation, and poration of seed coats. The cold atmospheric pressure He-plasma treatments of seeds produce hydrophilization of seed coats and improve the wetting properties of seed surfaces. Understanding mechanisms of plasma interactions with seeds and plants could promote plasma-based technology for plant developmental control, increasing yield, growth rates, and plant protection from pathogens. Our work offers new insight into mechanisms that trigger water transport and absorbance, seed germination, and activation of metabolism by cold plasmas. This work is supported by the NSF EPSCoR RII-Track-1 Cooperative Agreement OIA1655280. [Preview Abstract] |
Tuesday, October 6, 2020 3:30PM - 3:45PM Live |
KT4.00003: Effectiveness of cold plasma treatment during rice cultivation for growth and yield Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Susumu Nikawa, Masayoshi Maeshima, Masaaki Mizuno, Masaru Hori The applications of cold plasma in biological field, such as medicine and agriculture, have much attention. We previously showed that cancer cells were selectively killed by the direct irradiation and the indirect treatment such as plasma-activated Ringer’s lactate solution (PAL). On the basis of the approaches, in this study, we focused on the rice brewery cultivar and performed the plasma treatment on the plants with the direct irradiation or PAL solution twice a week during the cultivation in the University paddy field in Togo town, Aichi. After harvest, we measured the traits related to height, weight, and yield of the plants. The growth and yield from the plants with the direct irradiation were improved compared with the control ones. In the case of PAL treatment, the quality of brown rice as a trait of brewery cultivar was increased rather than the growth and yield. The results indicated that the plasma treatment in either way was effective for rice cultivation. [Preview Abstract] |
Tuesday, October 6, 2020 3:45PM - 4:00PM Live |
KT4.00004: Plasma activated Ringer's lactate solution affected cellular respiratory system on HeLa cells. Hiromasa Tanaka, Shogo Maeda, Kae Nakamura, Hiroshi Hashizume, Kenji Ishikawa, Mikako Ito, Kinji Ohno, Masaaki Mizuno, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori We have previously developed low temperature plasma with high electron density, and we have applied for cancer treatments. We found that plasma irradiated solutions exhibited anti-tumor effects, and we have further developed plasma-activated medium (PAM) and plasma-activated Ringer's lactate solution (PAL). We have investigated extracellular and intracellular reactive oxygen and nitrogen species (RONS) in PAL-treated HeLa cells, and we analyzed roles of extracellular hydrogen peroxide and the other components in PAL. We further investigated cellular respiratory system of PAL-treated HeLa cells using an extracellular flux analyzer and a probe to measure mitochondrial membrane potential, and we found that the plasma-activated lactates inhibited cellular respiratory system on HeLa cells. These findings shed light on a new mechanism that plasma-activated lactates induce cell death. This work was partly supported by a Grant-in-Aid for Specially Promoted Research (No. 19H05462) and a Grant-in-Aid for Scientific Research (C) (No. 18K03599) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. [Preview Abstract] |
Tuesday, October 6, 2020 4:00PM - 4:15PM Live |
KT4.00005: Proliferation promotion of fibroblast cells using atmospheric-pressure radical source. Naoyuki Iwata, Yuki Hori, Oh Jun-Seok, Tomiyasu Murata, Kenji Ishikawa, Masaru Hori, Masafumi Ito Recently, biological applications of atmospheric-pressure plasmas have been extensively researched. In this study, we investigated that the proliferation promotion of fibroblast cells in phosphate buffered saline (PBS) using a non-equilibrium radical source which selectively supplies electrically neutral radicals. The radical source was driven with Ar, O$_{\mathrm{2}}$ and N$_{\mathrm{2}}$ gases. The flow rate of Ar was fixed at 4 slm and the flow ratio of N$_{\mathrm{2}}$ against the sum of N$_{\mathrm{2}}$ and O$_{\mathrm{2}}$, hereafter N$_{\mathrm{2}}$/(N$_{\mathrm{2}}+$O$_{\mathrm{2}})$, was varied within 1 slm. The species and densities of supplied neutral radicals were investigated using a mass spectrometer (Hiden Analytical). As a result, N\textbullet , O\textbullet , NO\textbullet , NO$_{\mathrm{2}}$\textbullet and O$_{\mathrm{3}}$ were found to be major species. 3ml PBS with fibroblast cells were placed in a dish and the sample was treated for 10, 15, 20 and 30 s using the radical source with N$_{\mathrm{2}}$/(N$_{\mathrm{2}}+$O$_{\mathrm{2}})$ of 60, 70 and 80 {\%}. Cell viability was promoted compared to untreated samples with various exposure times and N$_{\mathrm{2}}$/(N$_{\mathrm{2}}+$O$_{\mathrm{2}})$, and the maximum promotion ratio was 34{\%} at a N$_{\mathrm{2}}$/(N$_{\mathrm{2}}+$O$_{\mathrm{2}})$ of 70{\%} and 15 s exposure. Also, the promotion ratio showed strong dependency only on the NO\textbullet dose. This result strongly suggests that NO\textbullet is the responsible factor for the proliferation promotion of fibroblast cells using atmospheric-pressure plasmas. [Preview Abstract] |
Tuesday, October 6, 2020 4:15PM - 4:30PM Live |
KT4.00006: Metabolomic profiles of glioblastoma in plasma-activated solutions Kenji Ishikawa, Hiromasa Tanaka, Li Jiang, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Camelia Miron, Masaru Hori Cell culture media and Ringer's lactate solution irradiated by non-thermal plasma are defined as plasma-activated medium (PAM) and plasma-activated lactate (PAL), respectively. By analysis of metabolomic profiles of a hundred intracellular metabolites using capillary electrophoresis mass spectrometry, glioblastoma cells U251SP cultivated in PAM and PAL were changes in intracellular metabolites. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway. The metabolomic profiles of the PAL-treated U251SP were changed with generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells even in reductive microenvironments than PAM. [Preview Abstract] |
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