Metabolomic profiles of glioblastoma in plasma-activated solutions*

Cell culture media and Ringer's lactate solution irradiated by non-thermal plasma are defined as plasma-activated medium (PAM) and plasma-activated lactate (PAL), respectively. By analysis of metabolomic profiles of a hundred intracellular metabolites using capillary electrophoresis mass spectrometry, glioblastoma cells U251SP cultivated in PAM and PAL were changes in intracellular metabolites. The metabolomic profiles of the PAM-treated U251SP cells were changed significantly with inhibition of the glycolysis pathway and with enhancement of the pentose phosphate pathway. The metabolomic profiles of the PAL-treated U251SP were changed with generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells even in reductive microenvironments than PAM.