54th Annual Meeting of the APS Division of Plasma Physics
Volume 57, Number 12
Monday–Friday, October 29–November 2 2012;
Providence, Rhode Island
Session PI2: Plasma Wall Interactions, Disruptions, and Plasma Technology
2:00 PM–5:00 PM,
Wednesday, October 31, 2012
Room: Ballroom DE
Chair: Michael Jaworski, Princeton Plasma Physics Laboratory
Abstract ID: BAPS.2012.DPP.PI2.5
Abstract: PI2.00005 : Cold Atmosphere Plasma in Cancer Therapy
4:00 PM–4:30 PM
Preview Abstract
Abstract
Author:
Michael Keidar
(George Washington University)
Plasma is an ionized gas that is typically generated in high-temperature
laboratory conditions. Recent progress in atmospheric plasmas led to the
creation of cold plasmas with ion temperature close to room temperature.
Areas of potential application of cold atmospheric plasmas (CAP) include
dentistry, drug delivery, dermatology, cosmetics, wound healing, cellular
modifications, and cancer treatment.
Various diagnostic tools have been developed for characterization of CAP
including intensified charge-coupled device cameras, optical emission
spectroscopy and electrical measurements of the discharge propertied.
Recently a new method for temporally resolved measurements of absolute
values of plasma density in the plasma column of small-size atmospheric
plasma jet utilizing Rayleigh microwave scattering was proposed [1,2]. In
this talk we overview state of the art of CAP diagnostics and understanding
of the mechanism of plasma action of biological objects.
The efficacy of cold plasma in a pre-clinical model of various cancer types
(long, bladder, and skin) was recently demonstrated [3]. Both in-vitro and
in-vivo studies revealed that cold plasmas selectively kill cancer cells. We
showed that: (a) cold plasma application selectively eradicates cancer cells
in vitro without damaging normal cells. For instance a strong selective
effect was observed; the resulting 60--70{\%} of lung cancer cells were
detached from the plate in the zone treated with plasma, whereas no
detachment was observed in the treated zone for the normal lung cells under
the same treatment conditions. (b) Significantly reduced tumor size in vivo.
Cold plasma treatment led to tumor ablation with neighbouring tumors
unaffected. These experiments were performed on more than 10 mice with the
same outcome. We found that tumors of about 5mm in diameter were ablated
after 2 min of single time plasma treatment. The two best known cold plasma
effects, plasma-induced apoptosis and the decrease of cell migration
velocity can have important implications in cancer treatment by localizing
the affected area of the tissue and by decreasing metastasic development. In
addition, cold plasma treatment has affected the cell cycle of cancer cells.
In particular, cold plasma induces a 2-fold increase in cells at the
G2/M-checkpoint in both papilloma and carcinoma cells at about 24 hours
after treatment, while normal epithelial cells (WTK) did not show
significant differences. It was shown that reactive oxygen species
metabolism and oxidative stress responsive genes are deregulated. We
investigated the production of reactive oxygen species (ROS) with cold
plasma treatment as a potential mechanism for the tumor ablation observed.
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[1] Shashurin A., Shneider M.N., Dogariu A., Miles R.B. and Keidar M. Appl.
Phys. Lett. (2010) \textbf{96}, 171502.\\[0pt]
[2] Shashurin A., Shneider M.N., Keidar M. Plasma Sources Sci. Technol. 21
(2012) 034006.\\[0pt]
[3]. M. Keidar, R. Walk, A. Shashurin, P. Srinivasan, A. Sandler, S.
Dasgupta , R. Ravi, R. Guerrero-Preston, B. Trink, British Journal of
Cancer, 105, 1295-1301, 2011
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2012.DPP.PI2.5