Bionanoparticle Characterization Using an Integrated Acoustofluidic Device with Raman Spectroscopy for Vaccine Development*

The impact of pandemics and viral outbreaks depends heavily on the speed of the vaccine development. Traditional vaccine production methods involve multiple stages and require manual human intervention, resulting in a long lead time. Developing reliable sensors and measurement methods for automated, real-time monitoring could significantly accelerate this process. Raman spectroscopy is a potential real-time measurement technique for determining the physical characteristics of samples. When dealing with bionanoparticles ranging from 50 nm to 400 nm in diameter, higher concentrations (more than 1E+9 particles/mL) are required. In this presentation, we will develop optimized acoustofluidics, demonstrating the practical application of the acoustofluidic device by successfully measuring Raman spectra from low-concentration Ebola vaccine. Our work offers an approach of real-time bionanoparticle monitoring through microfluidics, enabling the real-time quantification of vaccines.