Session Y45: Focus Session: Physics of Cancer II
8:00 AM–11:00 AM, Friday, March 22, 2013
Hilton Baltimore Room: Holiday Ballroom 4
Sponsoring Unit:
DBIO
Chair: Larry Nagahara, National Institutes of Health
Abstract ID: BAPS.2013.MAR.Y45.9
Abstract: Y45.00009 : The Causality of Evolution on Different Fitness Landscapes
10:00 AM–10:12 AM
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Abstract 
Authors:
Saurabh Vyawahare
(Princeton University)
Robert Austin
(Princeton University)
Qiucen Zhang
(University of Illinois CU)
Hyunsung Kim
(University California Santa Cruz)
John Bestoso
(Princeton University)
Evolution of antibiotic resistance is a growing problem. One major reason why most antibiotics fail is because of mutations on drug targets (e.g. essential enzymes). Sequencing of clinically resistant isolates have shown that multiple mutational-hotspots exist in coding regions, which could potentially prohibit the binding of drugs. However, it is not clear whether the appearance of each mutation is random or influenced by other factors. In this paper, we compare evolution of resistance to ciprofloxacin from two distinct but well characterized genetic backgrounds. By combining our recently developed evolution reactor and deep whole-genome sequencing, we show different alleles of $\sigma_s$ factor lead to fixation of different mutations in {\em gyrA} gene that confer ciprofloxacin resistance to bacteria {\em Escherichia coli}. Such causality of evolution in different genes provides an opportunity to control the evolution of antibiotic resistance.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2013.MAR.Y45.9