Session D21: Focus Session: Novel Instrumentation & Measurements for Biomedical Research
2:30 PM–5:30 PM, Monday, March 21, 2011
Room: D161
Sponsoring Unit:
GIMS
Chair: Larry Nagahara, National Institutes of Health
Abstract ID: BAPS.2011.MAR.D21.2
Abstract: D21.00002 : 3D Cell Culture Imaging with Digital Holographic Microscopy
3:06 PM–3:18 PM
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Abstract 
Authors:
Thomas Dimiduk
(Harvard University)
Kendra Nyberg
(University of Oregon)
Dariela Almeda
(Harvard University)
Ekaterina Koshelva
(University of Chigago)
Ryan McGorty
(Harvard University)
David Kaz
(Harvard University)
Emily Gardel
(Harvard University)
Debra Auguste
(Harvard University)
Vinothan Manoharan
(Harvard University)
Cells in higher organisms naturally exist in a three dimensional (3D) structure, a fact sometimes ignored by in vitro biological research. Confinement to a two dimensional culture imposes significant deviations from the native 3D state. One of the biggest obstacles to wider use of 3D cultures is the difficulty of 3D imaging. The confocal microscope, the dominant 3D imaging instrument, is expensive, bulky, and light-intensive; live cells can be observed for only a short time before they suffer photodamage. We present an alternative 3D imaging techinque, digital holographic microscopy, which can capture 3D information with axial resolution better than $2 \mu m$ in a $100\mu m$ deep volume. Capturing a 3D image requires only a single camera exposure with a sub-millisecond laser pulse, allowing us to image cell cultures using five orders of magnitude less light energy than with confocal. This can be done with hardware costing $\sim\$1000$. We use the instrument to image growth of MCF7 breast cancer cells and p. pastoras yeast.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2011.MAR.D21.2