Session P25: Focus Session: DNA and Protein Analysis with Micro and Nanofluidics

8:00 AM–11:00 AM, Wednesday, March 12, 2008
Morial Convention Center - 217

Sponsoring Units: DPOLY DBP
Chair: Ron Larson, University of Michigan

Abstract ID: BAPS.2008.MAR.P25.1

Abstract: P25.00001 : Learning from the Jersey Turnpike: Cell Lysis, Labeling and Washing with Microfluidic Metamaterials

8:00 AM–8:36 AM

Preview Abstract

Author:

  Robert Austin
    (Princeton University)

Directing objects across functional streamlines at low Reynolds number is difficult but important since this motion can be used to label, lyse, and analyze complex biological objects on-chip without cross-contamination. Here we use an asymmeteric post array to move cells across coflowing reagents and show on-chip, immunofluorescent labeling of platelets with washing and \emph{E.Coli} cell lysis with simultaneous separation of bacterial chromosome from the cell contents. Furthermore, we develop the concept of a microfluidic metamaterial by using the basic asymmetric post array as a building block for complex particle handling modes. These modular array elements could be of great use for developing robust techniques for on-chip, continuous flow manipulation and analysis of cells, large bio-particles, and functional beads.

To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2008.MAR.P25.1