NMR Spectroscopic Investigation of Aberrant Cancer Metabolism and Factors that Affect Nuclear Relaxation*

The accelerated glycolysis in glioblastoma was analyzed using nuclear magnetic resonance spectroscopy.  The rate of glucose metabolism and production of metabolites is increased to enhance cell proliferation in glioblastoma making it a useful biomarker in tracking carcinogenesis and cancer treatments with dynamic nuclear polarization.  We utilize nuclear magnetic resonance to determine characteristics about the physical and biochemical environment of specific molecules containing isotopes with magnetic spin. The effect of certain properties in the environment specifically viscosity, tumbling rate, and the presence of free electrons were reexamined.  We will present on how Ho-DOTA and 4-oxo-Tempo have ideal relaxivity for DNP enhancement and how we tracked aberrant glycolysis in brain cancer cells.