Metabolic Competition of Glucose and Mannose in SFXL Glioblastoma Cells*

Authors

Omar Raza

Department of Physics, University of Texas at Dallas, Richardson, Texas 75080

 

Lloyd Lumata, PhD

Department of Physics, University of Texas at Dallas, Richardson, Texas 75080

Cancer cells are known to have a voracious appetite for sugars and other nutrients to sustain their rapid growth and proliferation. In this project, two hexose sugars glucose and the structurally similar mannose were investigated as metabolic fuels for the proliferation and growth of SFXL glioblastoma cells. 10 mM [1-13C] glucose and 10 mM [1-13C] mannose were first administered individually in cultured SFXL glioblastoma cells under standard complete media incubated under normoxic conditions for 24 hrs. The metabolic competition of these sugars was examined by administering 10 mM [1-13C] glucose + 10 mM unlabeled mannose or 10 mM [1-13C] mannose + 10 mM unlabeled glucose in SFXL cells incubated for 24 hrs. Lactate productions from those four sugar sample preparations were compared and analyzed via nuclear magnetic resonance (NMR). The details of these NMR results will be discussed with regards to possible metabolic competition between the two sugars.