Bulletin of the American Physical Society
APS March Meeting 2024
Monday–Friday, March 4–8, 2024; Minneapolis & Virtual
Session S37: Microbial Communities III
8:00 AM–10:24 AM,
Thursday, March 7, 2024
Room: 103C
Sponsoring
Unit:
DBIO
Chair: Peter Yunker, Georgia Institute of Technology
Abstract: S37.00003 : Mechanical properties of Staphylococcus aureus and Pseudomonas aeruginosa dual-species biofilms grown in chronic wound based models*
8:48 AM–9:00 AM
Presenter:
Bikash F Bhattarai
(Texas Tech University)
Authors:
Gordon Christopher
(Texas Tech University)
Bikash F Bhattarai
(Texas Tech University)
Kendra P Rumbaugh
(Texas Tech University Health Science Campus)
This study builds on previous work, investigating how wound bed environment aids in the formation of polymicrobial biofilms, affects their mechanical stability, and ultimately impacts debridement. We hypothesize that collagen, a primary component in wound extracellular matrix, mediates between S. aureus and P. aeruginosa allowing formation of more robust polymicrobial biofilms. Using microrheology and colony counting, we have observed that biofilms grown in cultures that include collagen and secondary wound proteins form polymicrobial biofilms when both bacteria are initially present in culture. Furthermore, these biofilms are more elastic and stiffer in comparison to single species biofilms grown in identical conditions. However, if collagen is absent or collagen is present without a secondary protein, a single species biofilm will form from an initial co‑culture. These results indicate that the host environment plays an important role in mediating the formation of polymicrobial biofilms.
*Dr. Christopher gratefully acknowledge support from DOE/NNSA MSIPP Growing STEMS grant number DOE NA0003988 and Dr. Betsy Snell, Program Manager
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