Bulletin of the American Physical Society
APS March Meeting 2024
Monday–Friday, March 4–8, 2024; Minneapolis & Virtual
Session N00: Poster Session II (11:30am-2:30pm CST)
11:30 AM,
Wednesday, March 6, 2024
Room: Hall BC
Sponsoring
Unit:
APS/SPS
Abstract: N00.00280 : The Synthesis, Characterization, and Biocompatible Study of Boron Nitride Dots*
Presenter:
Raksha FNU
(Michigan Technological University)
Author:
Raksha FNU
(Michigan Technological University)
Collaborations:
Sambhawna Sharma, Raksha Dubey, Zohreh Salimi, Rodney Oakley, Xiuling Liu2, Nazmiye Yapici, Estele Chiari, Dongyan Zhang, Yoke Khin Yap
Fluorescent characterization techniques are useful for biomedical research as they offer cost-efficiency, molecular-scale sensitivity, and non-invasive nature for studying microbiological systems. Fluorescent dye or fluorophores used in these techniques have enabled the visualization, localization, and characterization of molecules within biological cells. Moreover, they play an important role in target detection, which helps in disease detection in their early stages.
Here we report the feasibility of using zero-dimensional boron nitride nanoparticles (BN dots) as a new class of fluorophores for biological application. Specifically, our BN dots are prepared by sonication-assisted solvothermal method, therefore we refer to them as ssBN dots herein. The synthesized ssBN dots are producing greenish fluorescence. We performed the bio-compatible study by incubating ssBN dots with HeLa cells at various concentrations. At concentrations lower than 10 μg/mL of ssBN dots, we observed continuous cell growth over four days. At 10 μg/mL of ssBN dots, we started to detect cell death on the fourth day. Trypan Blue assay and Pico-Green dsDNA assay were used to quantify cell proliferation. The results from the Pico-Green assay showed similar dsDNA concentrations between ssBN dots and the control samples after two days. The results suggest that ssBN dots possess good biocompatibility with HeLa cells at concentrations below 10 μg/mL. These results confirm the potential use of ssBN dots as fluorescent probes for biomedical applications and an alternative to traditional organic dyes with improved photophysical properties and biocompatibility. Further studies are needed to explore the full potential of ssBN dots and confirm their viability in a range of biomedical applications. The synthesis of ssBN dots, their properties revealed by atomic force microscopy (AFM), transmission electron microscopy (TEM), UV-Vis, and fluorometer, and the results of biocompatibility will be discussed in the meeting.
*Michigan Technological University, Department of PhysicsStabiLux Biosciences, Inc
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