Bulletin of the American Physical Society
APS March Meeting 2024
Monday–Friday, March 4–8, 2024; Minneapolis & Virtual
Session N00: Poster Session II (11:30am-2:30pm CST)
11:30 AM,
Wednesday, March 6, 2024
Room: Hall BC
Sponsoring
Unit:
APS/SPS
Abstract: N00.00135 : Genomic analysis in a solid state nanopore device using single-strand binding proteins*
Presenter:
Victor Corona
(CSULB)
Authors:
Alexander R Klotz
(California State University, Long Beach)
Victor Corona
(CSULB)
Nathan Howald
(CSULB)
The goal of this project is to develop a rapid genomics platform using solid state nanopores to detect denatured AT-rich sites on DNA. Using single strand binding protein, we aim to locate the binding sites on each DNA molecule passing through the nanopore by reading the current drop due to the protein. The nanopores used are created using controlled dielectric breakdown of a silicon nitride membrane with a 4M LiCl buffer solution to produce pores between 5 and 20nm for our experiments, and DNA is translocated through the pore by electrophoresis. Previously, we showed selective single strand protein binding to the ends of DNA molecules in a 30 nm pore with a noisy amplifier. In this work we have improved the sensitivity of the device and decreased the size of the pores to allow more precise measurements, and explored a wider parameter space to facilitate binding to sequence specific denaturation bubbles.
*NSF DMR-BMAT 2105113
Follow Us |
Engage
Become an APS Member |
My APS
Renew Membership |
Information for |
About APSThe American Physical Society (APS) is a non-profit membership organization working to advance the knowledge of physics. |
© 2024 American Physical Society
| All rights reserved | Terms of Use
| Contact Us
Headquarters
1 Physics Ellipse, College Park, MD 20740-3844
(301) 209-3200
Editorial Office
100 Motor Pkwy, Suite 110, Hauppauge, NY 11788
(631) 591-4000
Office of Public Affairs
529 14th St NW, Suite 1050, Washington, D.C. 20045-2001
(202) 662-8700