Bulletin of the American Physical Society
2024 APS March Meeting
Monday–Friday, March 4–8, 2024; Minneapolis & Virtual
Session M35: Emerging Trends in Soft Microscale Mechanics I
8:00 AM–10:48 AM,
Wednesday, March 6, 2024
Room: 103A
Sponsoring
Unit:
DSOFT
Chair: Rae Robertson-Anderson, University San Diego
Abstract: M35.00008 : Optical tweezers map spatiotemporal force generation in active actin-microtubule composites*
9:48 AM–10:00 AM
Presenter:
Anindya Chowdhury
(University Of San Diego)
Authors:
Anindya Chowdhury
(University Of San Diego)
Karthik Reddy Peddireddy
(University of San Diego)
Moumita Das
(Rochester Institute of Technology)
Mehrzad Sasanpour
(University of San Diego)
Jennifer L Ross
(Syracuse University)
Ryan J McGorty
(University of San Diego)
Megan T Valentine
(University of California, Santa Barbara)
Michael J Rust
(University of Chicago)
Rae M Robertson-Anderson
(University San Diego)
The cytoskeleton is a dynamic network of proteins, including actin, microtubules, and their associated motor proteins that enables essential cellular processes such as motility, division, and growth. Previous study has shown co-entangled actin-microtubule networks driven by myosin exhibit organized and controlled contraction dynamics. In contrast, similar actomyosin networks that lack microtubules undergo fast, multidirectional motion and network rupturing. However, the underlying motor-generated forces that give rise to these different behaviors is poorly understand. Here, we combine optical tweezers microrheology, fluorescence microscopy and photo-controlled myosin activity, to measure local contractile forces in active co-entangled actin-microtubule composites. By measuring the forces on beads that are held fixed at different positions throughout the network, with a particular focus on force generation at the network boundary, we determine the stress that the composites can self-generate, and how these stresses propagate through the network from the contractile edge. By simutaneously imaging the actively restructuring filaments in the composite we directly correlate structure and deformation fields of the network to force generation.
*NSF-DMREF-2119663, NIH NIGMS R15GM123420
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