Reusable Surface Amplified Nanobiosensor for the Detection of Airborne Virus

We reported a reusable surface-amplified nano biosensor for monitoring airborne viruses with a sub-PFU/mL detection limit. In this work, sandwich structures were formed by attaching target virus molecules to antibody-functionalized magnetic particles and alkaline phosphatase (ALP) enzyme. Then, electrochemical markers were generated by ALPs trapped on the Ni patterns by the external magnetic field, resulting in signal amplification. We detected target hemagglutinin (HA) of influenza A (H1N1) virus down to 10 aM concentration using a single sensor chip. Even after repeated sensing measures over the 18 times, the sensor maintained a consistent sensing signal. Moreover, airborne influenza viruses collected from the air could be measured down to sub-PFU/mL level. Considering the reusability and ultrasensitivity of our sensors, it could be a powerful tool for the monitoring of airborne pathogens and help to prevent epidemics by airborne pathogens.