Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session W04: Single-Molecule Characterization in Polymers and Soft Matter: Topology and Transport
3:00 PM–5:48 PM,
Thursday, March 9, 2023
Room: Room 127
Sponsoring
Unit:
DPOLY
Chair: Danielle Mai, Stanford University; Alexander Klotz
Abstract: W04.00013 : Nanopore tomography for quantitative assessment of geometrical parameters of filamentous fd bacteriophage*
5:24 PM–5:36 PM
Presenter:
Arjav Shah
(Massachusetts Institute of Technology MIT)
Authors:
Arjav Shah
(Massachusetts Institute of Technology MIT)
Kun Li
(Singapore-MIT Alliance for Research and Technology Centre, Singapore)
Slaven Garaj
(Department of Physics, National University of Singapore, Singapore)
Patrick S Doyle
(Massachusetts Institute of Technology MIT)
Collaborations:
MIT, Singapore-MIT Alliance for Research and Technology Centre (SMART)
In this work, we apply NT methodology to measure the diameter of fd, a long, filamentous bacteriophage translocating through a solid-state nanopore at high salt concentrations. The diameter of the virus is subject to inference from experimentally reported changes in current. Continuum simulations are performed to estimate the phage diameter from these current blockades. Furthermore, the robustness and sensitivity of these estimates to variations in the physicochemical and geometrical parameters of the system are investigated. In addition, we present and benchmark helium ion microscopy (HIM) as an alternative imaging technique superior to widely used TEM and AFM.
The calculated diameters match closely with the measurements using standard imaging techniques. The methodology is high throughput, requires minimal sample preparation, and performs favorably as compared to the other standard imaging techniques. In conclusion, this study serves as a proof-of-concept for the use of NT to characterize small viruses.
*This work was supported by National Research Foundation, Prime Minister’s Office, Singapore and Singapore-MIT Alliance for Research and Technology Centre (SMART).
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