Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session Q07: Mechanisms of Self-Assembly: Biology and Beyond I
3:00 PM–5:48 PM,
Wednesday, March 8, 2023
Room: Room 130
Sponsoring
Units:
DSOFT DPOLY DBIO
Chair: Jonathan Whitmer, University of Notre Dame; Samanvaya Srivastava, UCLA
Abstract: Q07.00011 : Dynamically tuning the membrane lipid composition can control macromolecular assembly*
5:00 PM–5:12 PM
Presenter:
Jonathan A Fischer
(Johns Hopkins University)
Author:
Jonathan A Fischer
(Johns Hopkins University)
into the cell, requiring hundreds of protein components to assemble into
large macromolecular structures on the cell membrane. The composition of
the plasma membrane is critical for initiating assembly, as proteins bind
specifically only to subpopulations of lipids. Two opposing lipid-modifying
enzymes can dynamically tune these lipid populations, and it is not known to
what extent these enzymes help promote or inhibit protein recruitment at
specific places and times. This dynamical complexity of CME makes it not
only an interesting process to study for its own sake, but for the potential to
reveal general principles for controlling self-assembly on a 2D surface.
However, this very complexity and the fast time scales make CME difficult to
interrogate in vivo with sufficient resolution, thus the relationships between
environmental variables and the dynamics of clathrin-coated vesicle
formation are yet to be fully characterized. Here, we use computational
modeling to quantify how macromolecular self-assembly can be dynamically
tuned via the composition of the plasma membrane and protein
stoichiometry. Our models solve differential equations with the structure-
resolved reaction-diffusion software NERDSS, as well as non-spatial ordinary
differential equations that are amenable to efficient parameter optimization
and sensitivity analysis. We found a novel mechanism for biochemical
feedback loops allowing for switch-like lipid remodeling and oscillatory
behavior. We discuss how the principles established here for controlling
membrane composition are informative for a broader range of processes
across biological systems.
*NIH MIRA
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