Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session N00: Poster Session II (11am-2pm PST)
11:00 AM,
Wednesday, March 8, 2023
Room: Exhibit Hall (Forum Ballroom)
Sponsoring
Unit:
APS
Abstract: N00.00345 : Bacterial replication initiation as precision control in biology
Presenter:
Haochen Fu
(UC San Diego)
Authors:
Haochen Fu
(UC San Diego)
Dongyang Li
(University of California, San Diego)
Suckjoon Jun
(University of California, San Diego)
Fangzhou Xiao
(Caltech)
Replication initiation in bacteria is one of the most precise physiological controls in biology. Curiously, E. coli produces more than one order of magnitude larger number of DnaA proteins than needed, the widely conserved master regulator proteins in bacteria. More than 90% of the DnaA proteins are titrated by 300 binding sites encoded in the chromosome known as the DnaA boxes. In this work, we demonstrate that the number of DnaA boxes is optimal for initiation noise reduction and far more effective than autoregulation of DnaA production. We developed several novel experimental methods to control the number of DnaA boxes. As we increase the number of DnaA boxes, initiation is delayed steadily. However, the initiation noise (measured by CV) shows a non-monotonic behavior with the minimum at the wild-type number. We propose a model based on the DnaA flux to explain our results. We further discuss general implications of our results for precision control in biology.
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