Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session G14: Bio-inspired Phase Separation I
11:30 AM–2:30 PM,
Tuesday, March 7, 2023
Room: Room 206
Sponsoring
Units:
DSOFT DPOLY
Chair: Omar Saleh, University of California, Santa Barbara; Sam Wilken, University of California, Santa Barbara
Abstract: G14.00010 : Modular RNA motifs for orthogonal phase-separated compartments*
1:42 PM–1:54 PM
Presenter:
Shiyi Li
(UCLA)
Authors:
Elisa Franco
(UCLA)
Shiyi Li
(UCLA)
Anli Tang
(UCLA)
Jaimie M Stewart
(Caltech)
Melissa A Klocke
(University of California, Riverside)
Paul Rothemund
(Caltech)
Phase separation of biomacromolecules into immiscible membrane-less organelles contributes to regulating distinct biochemical functions. However, re-enacting and controlling such orthogonality remains a challenge. In this work, we constructed pure-RNA condensates from single-stranded motifs (monomers) and verified their ability to co-exist as orthogonal compartments that were tracked with the addition of multicolor fluorogenic RNA aptamers. The RNA monomers, which fold into star-shaped structures, are designed to interact through HIV-typed kissing loops and generate different droplet morphologies based on the sequence designs. Furthermore, we demonstrated that these single-stranded nanostars can form droplet-like condensates cotranscriptionally in vitro in physiological conditions. Our work showcases strategies to build a highly programmable library of orthogonal RNA assemblies facilitating the understanding of the spatial organization of bioprocess and offers a potential tool to engineer artificial organelles within living cells.
*NSF CAREER award 1938194; FMRG: Bio award 2134772; Sloan Foundation award G-2021-16831
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