Bulletin of the American Physical Society
APS March Meeting 2023
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session B10: Quantitative Cell Physiology I - Shape and Size
11:30 AM–2:30 PM,
Monday, March 6, 2023
Room: Room 202
Sponsoring
Unit:
DBIO
Chair: Suckjoon Jun, University of California, San Diego
Abstract: B10.00009 : Direct measurement of turgor pressure in E. coli and B. subtilis*
1:06 PM–1:18 PM
Presenter:
Octavio Albarran
(Duke University)
Authors:
Octavio Albarran
(Duke University)
Christoph F Schmidt
(Duke University)
renata garces
(Duke University)
Jeff D Eldredge
(University of California, Los Angeles)
Harold P Erickson
(Duke University)
proper metabolism and growth in walled microbial cells. To date, turgor pressure has only been estimated or
indirectly measured, because direct access has been difficult due to small cell sizes. How turgor pressure
depends on external conditions such as osmolarity and nutrient content remains largely unknown. Here we
present a micromanipulation technique using an atomic force microscope (AFM) that allows us to directly
measure and track turgor pressure on a living bacterium. We compress single cells between a large bead and a
flat surface using an AFM cantilever. Measured forces and indentation depths are then used to determine turgor
pressure with the help of a mechanical model that describes the deformation of the cell. We report dependencies
on external osmolarities for E. coli and B. subtilis.
*This work is funded by the National Science Foundation, award MCB-2221771-01.
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