Bulletin of the American Physical Society
APS March Meeting 2022
Volume 67, Number 3
Monday–Friday, March 14–18, 2022; Chicago
Session Y31: Biological Physics
8:00 AM–10:12 AM,
Friday, March 18, 2022
Room: McCormick Place W-192A
Sponsoring
Unit:
DBIO
Chair: Margaret Cheung, PNNL
Abstract: Y31.00002 : Epithelial-to-mesenchymal transition proceeds through directional destabilization of multidimensional attractor*
8:12 AM–8:24 AM
Presenter:
Weikang Wang
(University of Pittsburgh)
Authors:
Jianhua Xing
(University of Pittsburgh)
Weikang Wang
(University of Pittsburgh)
Dante Poe
(Univeristy of Pittsburgh)
Yaxuan Yang
(University of Pittsburgh)
Thomas Hyatt
(University of Pittsburgh)
Epithelial-to-mesenchymal transition (EMT) is a phenotypic transition process extensively studied recently but mechanistic details remain elusive. Through time-lapse imaging we recorded single cell trajectories of human A549/Vim-RFP cells undergoing EMT induced by different concentrations of TGF-β in a multi-dimensional cell feature space. The trajectories cluster into two distinct groups, indicating that the transition dynamics proceeds through parallel paths. We then reconstructed the reaction coordinates and corresponding pseudo-potentials from the trajectories. The potentials reveal a plausible mechanism for the emergence of the two paths as the original stable epithelial attractor collides with two saddle points sequentially with increased TGF-β concentration, and relaxes to a new one. Functionally the directional saddle-node bifurcation ensures a CPT proceeds towards a specific cell type, as a mechanistic realization of the canalization idea proposed by Waddington.
*This work was partially supported by National Cancer Institute (R37 CA232209), and National Institute of Diabetes and Digestive and Kidney Diseases (R01DK119232) to JX.
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