Anti-SARS-Cov-2 infection drugs and neutralizing antibody screening by palladium nano-thin-film electrode-based biosensor*

Outbreak of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) has infected more than 240 million people and caused nearly five million death. In order to alleviate the symptoms of viral infections and severe illness, drugs/functional peptides to suppressed the virus infection are highly desired. A rapid and sensitive anti-viral infection drugs screening sensing system has been developed by mimicking the SARS-CoV-2 enters the cell model. Four potential leads have been identified, such as perindoprilat and ramiprilat, which can reduce virus infection 67% and 72%, respectively by palladium nano-thin-film electrode-based biosensor (Pd-NTF biosensor) within 21 mins and at low analyte concentration and small volume (0.1 μg/mL and ~1 μL, estimated total analyte consumption < 4 pg). Furthermore, the titer of neutralizing antibody can be analyzed and the effective titer is as high as 19500.