Bulletin of the American Physical Society
68th Annual Gaseous Electronics Conference/9th International Conference on Reactive Plasmas/33rd Symposium on Plasma Processing
Volume 60, Number 9
Monday–Friday, October 12–16, 2015; Honolulu, Hawaii
Session WF4: Biomedical Applications |
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Chair: Deborah O'Connell, University of York Room: 303 AB |
Friday, October 16, 2015 3:30PM - 4:00PM |
WF4.00001: Plasma Polymers for Biomedical Applications Invited Speaker: Farzaneh Arefi There exists an abundant literature on polymers used for biomedical applications. However, the research described in the present talk deals with the plasma (co-) polymerization of different organic precursors for surface modifications of a variety of substrates in order to tailor the surface physico-chemical properties for tuneable biomolecule-surface interactions required for a wide range of biomedical applications such as antifouling properties, controlled drug delivery systems (DDS), cell-surface interactions for tissue engineering applications, etc. A low pressure inductively excited plasma, and a custom made open air DBD APPJ have been used for the plasma (co-) polymerization. Furthermore with the help of a coaxial double tube configuration of APPJ, one can minimize the influence of the entrainment of air in an open-air system in order to avoid enhanced plasma fragmentation and loss of the retention of the functional groups of the precursors, which is usually required for biomedical applications. The stability to washing with water and PBS of the deposited organic plasma polymers obtained with the APPJ at two different frequencies i.e. at 18 kHz and at 13.56 MHz will be compared. Examples such as multilayered DDS obtained from the plasma copolymerization of PCL-PEG coatings on collagen for controlled release of carboplatin for anticancer therapies and \textit{in-vitro} experiments will be presented. In the near future, DDS loaded with carboplatin will be tested \textit{in-vivo} on mice infected with mouse colon cancer CT26 and ovarian cancer cells OVCAR-3. Another example presented in the talk will be the deposition of biocompatible biodegradable PEG-PCL copolymers on Calcium Phosphates (CaPs) scaffolds, which are suitable biomaterial for bone regeneration materials, in order to control the kinetics of drug release. [Preview Abstract] |
Friday, October 16, 2015 4:00PM - 4:30PM |
WF4.00002: Diagnostics of Nonthermal Atmospheric Pressure Plasma for Plasma Biosciences and their Biological Cell Interactions throughout Ultraviolet Photolysis Invited Speaker: Eun Ha Choi Nonthermal biocompatible plasma (bioplasma) sources and their characteristics operating at atmospheric pressure have been introduced for biological cell interactions, especially used in Plasma Bioscience Research Center (PBRC), Korea. The electron temperatures and plasma densities are measured and analysed here for the nonthermal bioplasma sources in PBRC. Herein, we introduced plasma-initiated ultraviolet photolysis of water inside the biological solutions, to generate the reactive hydroxyl radical OH and hydrogen peroxide H$_{2}$O$_{2}$ species that may results in apoptotic cell death. These molecular changes in genomic DNA have been investigated by the confocal Raman and circular dichroism spectroscopy. We also found enhanced anticancer effect of monocytes and macrophages activated by nonthermal plasma which act as immune-modulator on these immune cells. Further, we investigated the action of the microsecond pulsed plasma activated media (MPP-AM) action on the lung cancer cells and its DNA oxidation pathway. Moreover, we also checked the action of heavy water and normal water activated water on the different cancer cell lines, to show the apoptotic cell death.\\[4pt] In collaboration with Young Joon Hong, Pankaj Attri, Naresh Kumar, and Nagendra Kaushik, Kwangwoon University. [Preview Abstract] |
Friday, October 16, 2015 4:30PM - 4:45PM |
WF4.00003: Monolithic Structure of Integrated Coaxial Micro-hollow Dielectric Barrier Discharges: Characterization for Environmental and Biomedical Application Kunihide Tachibana, Hideki Motomura Dielectric barrier discharge (DBD) devices operated at atmospheric pressure are of great uses for varieties of environmental and biomedical applications. Instead of commonly used parallel plate structures we have ever developed an alternative structure of integrated coaxial micro-hollow discharges by stacking two ceramic insulated metal meshes, which can be scaled to any size keeping uniform discharge. Since its new version of monolithic structure became available, we tried in this work to characterize the properties of discharge and production of OH radicals with several gas species using optical emission and laser induced fluorescence (LIF) spectroscopy techniques. Spatiotemporal behavior of DBD showed ring structures at larger hole-diameters than 1 mm but changed to convex structures at 0.6 mm hole-diameter. Large amounts of OH radicals were produced with He and Ar gases but almost none with N$_{2}$ gas. Spatial distribution of OH radicals diagnosed by LIF showed the propagation with the gas flow. Rotational temperature estimated from OH (0-0) band was larger than those from N$_{2}$$^{+}$ (0-0) and (0-1) bands. [Preview Abstract] |
Friday, October 16, 2015 4:45PM - 5:00PM |
WF4.00004: Localized Gene Transfection through Cell Membrane Stimulated by Micro Solution Plasma Toshiro Kaneko, Yutaro Hokari, Shota Sasaki, Makoto Kanzaki, Takehiko Sato The micro-scale plasmas generated in solution (micro solution plasmas) are strongly desired to realize in vivo gene transfection, because most of the human body consists of water and internal organs are filled with solution. We attempt to generate the micro solution plasma and apply it to the living cells for clarifying the transfection mechanism toward developing minimally-invasive localized gene transfection. In this experiment, the coaxial type electrode is used to make the micro-scale plasma, where the curvature radius of the high voltage electrode is less than 1 $\mu $m. By applying a pulse voltage to the electrode, we succeed in generating the micro-scale plasma in phosphate buffered saline (PBS), which could stimulate the adherent cells in PBS. After the micro solution plasma irradiation, the cell membrane permeability is evaluated using fluorescent probe YOYO-1 [1,2]. The YOYO-1 fluorescence is strongly observed only in the localized plasma irradiation area which can be controlled by the pulse width. Based on this result, the cell membrane permeability is found to be locally enhanced by the stimulation of the irradiated micro solution plasma. [1] S. Sasaki, et al., Appl. Phys. Exp., 7 (2014) 026202. [2] T. Kaneko, et al., Biointerphases, 10 (2015) 029531. [Preview Abstract] |
Friday, October 16, 2015 5:00PM - 5:15PM |
WF4.00005: Acquisition of Cell-Adhesion Ability on the Surface of Crosslinked Albumin Films Irradiated with Atmospheric-Pressure Plasma Jets Mami Iwamura, Kota Nakajima, Ryosuke Taga, Kenji Tanaka, Tatsuru Shirafuji, Akira Tachibana, Toshizumi Tanabe We have applied an atmospheric-pressure plasma jet (APPJ) using He gas to surface treatment on crosslinked albumin films. The crosslinked albumin films, to which L929 cells do not attach, acquire the L929 cell-adhesion ability by the APPJ irradiation in a quite short time of 300 s. The processing speed by the APPJ is 40-fold faster than a conventional ultraviolet light irradiation process. Furthermore, the elongated spindle-like morphology of the cells indicates strong adhesion between the cells and the film. We have confirmed the formation of hydrophilic chemical bonds such as COH and COOH through X-ray photoelectron spectroscopy (XPS) on the APPJ-irradiated crosslinked albumin films. An increase in the cell-adhesion ability with increasing the APPJ irradiation time has a positive correlation with the increase in O (1s) peak intensity in the XPS spectra of the films. However, the optical emission spectrum of the APPJ does not show strong emission of O (777 nm) and OH (309 nm). These results suggest that the causes of the hydrophilic chemical bonds are the dangling bond formation by abundant excited N$_2$ and He, and successive oxidation of the dangling bonds by ambient air and/or water vapor. [Preview Abstract] |
Friday, October 16, 2015 5:15PM - 5:30PM |
WF4.00006: Measurement of the diffusion coefficient of supported lipid bilayer irradiated with dielectric barrier discharge Yoshiyuki Suda, Ryuma Yamashita, Kota Yusa, Toru Harigai, Hirofumi Takikawa, Akinori Oda, Ryugo Tero We have focused on the behavior of artificial cell membrane system at solid-liquid interface. We irradiated the Ar or He gas dielectric barrier discharge (DBD) onto a supported lipid bilayer (SLB) [1-2]. Observation with a fluorescence microscope and atomic force microscope revealed the formation of pores on the order of 10 nm--1 $\mu $m in size without a change in pH. We propose that SLBs are effective for obtaining information about the physical and chemical modification of cell membranes induced by plasma. SLB was produced in buffer solution on the SiO$_{2}$ / Si substrate using the vesicle fusion method. DOPC (dioleoylphosphatidylcholine) and Rb-DOPE (rhodamine B-dioleoylphosphatidylethanolamine) were used as a lipid and fluorescent dye-labeled lipid, respectively. The diffusion coefficients of the SLBs before and after the He gas DBD irradiation were measured using a confocal laser scanning microscopy. It was found that the diffusion coefficient became 30{\%} lower after the DBD irradiation.\\[4pt] [1] Ryugo Tero, Yoshiyuki Suda, Ryo Kato, Hideto Tanoue, and Hirofumi Takikawa: Appl. Phys. Exp. \textbf{7} (2014) 077001 (4 pages);\\[0pt] [2] Yoshiyuki Suda, Akinori Oda, Ryo Kato, Ryuma Yamashita, Hideto Tanoue, Hirofumi Takikawa, and Ryugo Tero: Jpn. J. Appl. Phys. \textbf{54} (2015) 01AF03 (6 pages). [Preview Abstract] |
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