Bulletin of the American Physical Society
Four Corners Section 2022 Meeting
Volume 67, Number 14
Friday–Saturday, October 14–15, 2022; Albuquerque, New Mexico
Session J05: Biophysics and Soft Condensed Matter II
8:30 AM–10:06 AM,
Saturday, October 15, 2022
UNM
Room: PAIS 2540
Chair: Keith Lidke, The University of New Mexico
Abstract: J05.00005 : Extended X-ray Absorption Fine Structure Calibrated Hemoglobin-Bound Iron Fraction via Blood Thin Films Rapidly Solidified From 100µL Drops*
9:18 AM–9:30 AM
Presenter:
Arjun Sekar
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Authors:
Arjun Sekar
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Rianna N Rane
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Aarush Thinakaran
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Ashwin Suresh
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Pranav Penmatcha
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
Jordan Bischoff
(Simon Fraser Univ. Dept. of Physics)
Karen L Kavanagh
(Simon Fraser Univ. Dept. of Physics)
Nicole Herbots
(Arizona State Univ. Dept. of Physics/SiO2 Innovates)
In this work, small volume blood drops are rapidly solidified via Hyper-hydrophilic coatings [2] to yield flat, Homogeneous Thin Solid Films (HTSFs) in min. without coagulation. X-ray Fluorescence (XRF) and Ion Beam Analysis [2] yield Fe composition within ± 10% on HTSF.
Extended X-ray Absorption Fine Structure (EXAFS) can be used since solidifying blood into HTSFs does not modify Fe bonding.
Hemoglobin is a globular protein, folded into a spherical shape with four heme groups, each with an Fe bound to a histidine residue via N. Fe is bound to four N nearest neighbors within a plane, with a 5th nearest neighbor and a 6th N atom. Prior EXAFS on hemoglobin detected oxidized Fe, matching HPLC [1]. In this work, EXAFS at the Canadian Light Source uses fluorescence near the Fe absorption edge (7.126 -7.8 keV). Spectra on whole blood HTSF match [1] with a Fe-N bond length of 1.97±0.02 Å [1].
[1] Z. Wu et al.. (2001) Phys. Med. Biol, 46(71)
[2] Herbots, N., et al. (2019). MRS Adv.,4(46-47), 2489-2513. doi:10.1557/adv.2019.398
*CLS, NSERC, Matthew Newville, SiO2 Innovates
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