77th Annual Meeting of the Southeastern Section of the APS
Volume 55, Number 10
Wednesday–Saturday, October 20–23, 2010;
Baton Rouge, Louisiana
Session JA: Microfluidics: Computational and Experimental Challenges
3:45 PM–5:45 PM,
Friday, October 22, 2010
Nicholson Hall
Room: 119
Chair: Michael Martin, Louisiana State University
Abstract ID: BAPS.2010.SES.JA.2
Abstract: JA.00002 : Quantized Concentration Gradient in Picoliter Scale
4:15 PM–4:45 PM
Preview Abstract
Abstract
Author:
Jong Wook Hong
(Auburn University)
Generation of concentration gradient is of paramount importance
in the success of reactions for cell biology, molecular biology,
biochemistry, drug-discovery, chemotaxis, cell culture,
biomaterials synthesis, and tissue engineering. In conventional
method of conducting reactions, the concentration gradients is
achieved by using pipettes, test tubes, 96-well assay plates, and
robotic systems. Conventional methods require milliliter or
microliter volumes of samples for typical experiments with
multiple and sequential reactions. It is a challenge to carry out
experiments with precious samples that have strict limitations
with the amount of samples or the price to pay for the amount. In
order to overcome this challenge faced by the conventional
methods, fluidic devices with micrometer scale channels have been
developed. These devices, however, cause restrictions on changing
the concentration due to the fixed gradient set based on fixed
fluidic channels.\footnote{Jambovane, S.; Duin, E. C.; Kim, S-K.;
Hong, J. W., Determination of Kinetic Parameters, $K_{M}$ and
$k_{cat}$, with a Single Experiment on a Chip. textit{Analytical
Chemistry, }81, (9), 3239-3245, 2009.}$^,$\footnote{Jambovane,
S.; Hong, J. W., Lorenz-like Chatotic System on a Chip In
\textit{The 14th International Conference on Miniaturized Systems
for Chemistry and Life Sciences (MicroTAS)}, The Netherlands,
October, 2010.} Here, we present a unique microfluidic system
that can generate quantized concentration gradient by using
series of droplets generated by a mechanical valve based
injection method.\footnote{Jambovane, S.; Rho, H.; Hong, J.,
Fluidic Circuit based Predictive Model of Microdroplet Generation
through Mechanical Cutting. In \textit{ASME International
Mechanical Engineering Congress {\&} Exposition}, Lake Buena
Vista, Florida, USA, October, 2009.}$^,$\footnote{Lee, W.;
Jambovane, S.; Kim, D.; Hong, J., Predictive Model on Micro
Droplet Generation through Mechanical Cutting.
\textit{Microfluidics and Nanofluidics, }7, (3), 431-438, 2009.}
\textbf{Acknowledgement:} All this work has been done by Sachin
Jambovane, Kirn Cramer, Woon Seob Lee, and Hoon Suk Rho. The
presenter would like to thank them.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2010.SES.JA.2