Bulletin of the American Physical Society
2023 APS March Meeting
Volume 68, Number 3
Las Vegas, Nevada (March 5-10)
Virtual (March 20-22); Time Zone: Pacific Time
Session Y11: Morphogenesis II
8:00 AM–11:00 AM,
Friday, March 10, 2023
Room: Room 203
Sponsoring
Unit:
DBIO
Chair: Maryam Kohram, Princeton University
Abstract: Y11.00011 : Recording symmetry breaking during gastruloid morphogenesis with recombinase circuits*
10:24 AM–10:36 AM
Presenter:
Harold M McNamara
(Princeton University)
Authors:
Harold M McNamara
(Princeton University)
Jared Toettcher
(Princeton University)
We study symmetry breaking in the gastruloid model of axial morphogenesis using genetic circuits recorders of signaling histories. Recombinase circuits encode permanent labels of cells' signaling states within a ‘listening window’ defined through addition of the small molecule doxycycline. Clonal mouse embryonic cell lines expressing morphogen recorders can achieve high fidelity labeling with temporal resolution between 1 and 3 hours. By sampling a Wnt morphogen recorder at different timepoints during gastruloid morphogenesis, we map how dynamics of Wnt signaling encode anterior-posterior positional information. These measurements localize the timepoint of Wnt symmetry breaking to within a 6 hour time window. Notably, this window occurs before any apparent polarization in either gastruloid morphology or Wnt signaling domains, suggesting a key role for cellular rearrangements in axial morphogenesis. In ongoing work, we are usings morphogen recorders to trace signaling lineages across cell types using single-cell transcriptomics.
*This work was supported by the Lewis-Sigler Scholars program and the Center for Physics of Biological Function (PHY–1734030).
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