Bulletin of the American Physical Society
APS March Meeting 2021
Monday–Friday, March 15–19, 2021; Virtual; Time Zone: Central Daylight Time, USA
Session F02: Polyelectrolyte Complexation I
11:30 AM–2:30 PM,
Tuesday, March 16, 2021
Sponsoring Units: DPOLY DSOFT
Chair: Samanvaya Srivastava, University of California; Debra Audus, National Institute of Standards of Technology
Abstract: F02.00005 : Concentration and separation of proteins using polyion condensates*
12:42 PM–1:18 PM
The cellular fluids contain liquid-like domains with similar properties to polyion condensates. This is not surprising since these cellular structures consist of intrinsically disordered proteins (IDPs) which are zwitterionic in nature, these readily phase separate in vitro, or contain RNA, which is negatively charged, and IDPs with high amount of basic residues. These cellular condensates are expected to selectively partition other biomacromolecules.
Cellular condensates are very complex multi-component systems, which can be mimicked using simple polyion condensates, consisting of synthetic oppositely charged polyelectrolytes. In this talk I will discuss two features of these polyion condensates which can be linked to the cellular condensates. First of all their ability to concentrate proteins and second that the partitioning of proteins can be very selective. This selectivity can be used to separate two types of proteins. Both these features are also important for extraction processes in chemical engineering. This talk will therefore conclude by discussing the possibility to use these condensates as tunable extraction media for biorefinery applications.
*NWO VENI (# 722.013.013) and NWO VIDI (# 723.015.003)
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