Bulletin of the American Physical Society
APS March Meeting 2021
Volume 66, Number 1
Monday–Friday, March 15–19, 2021; Virtual; Time Zone: Central Daylight Time, USA
Session A12: Macromolecular Phase Separation I
8:00 AM–11:00 AM,
Monday, March 15, 2021
Sponsoring
Units:
DBIO DPOLY GSNP DSOFT
Chair: Daphne Klotsa, Univ of NC - Chapel Hill; Patrick McCall, Max Planck Institute
Abstract: A12.00006 : Quantitative Theory for the Diffusive Exchange Dynamics of Liquid Condensates
9:24 AM–9:36 AM
Live
Presenter:
Lars Hubatsch
(Max Planck Institute for the Physics of Complex Systems)
Authors:
Lars Hubatsch
(Max Planck Institute for the Physics of Complex Systems)
Louise Jawerth
(Max Planck Institute for the Physics of Complex Systems)
Celina Love
(Max Planck Institute for Cell Biology and Genetics)
Jonathan Bauermann
(Max Planck Institute for the Physics of Complex Systems)
TY Dora Tang
(Max Planck Institute for Cell Biology and Genetics)
Stefano Bo
(Max Planck Institute for the Physics of Complex Systems)
Anthony A Hyman
(Max Planck Institute for Cell Biology and Genetics)
Christoph Weber
(Max Planck Institute for the Physics of Complex Systems)
Using the theory of phase separation we derive a framework that quantitatively captures the diffusive transport of labeled droplet components. Based on our framework, we analyse FRAP experiments and show that diffusion coefficients inside liquid droplets can be precisely determined by combining experimentally measured concentrations at the droplet interface with the governing equation inside of liquid droplets.
We proof the accuracy of this method by considering space and time resolved FRAP data of PGL-3 droplets as well as two different coacervate systems. Strikingly, without explicitly measuring the outside dynamics, we can also determine the outside diffusion coefficient or the partitioning.
Thus, by combining the theory of phase separation and dynamic measurements of concentration fields, we provide a framework to characterize physical properties highly relevant to understand condensate functions.
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