Bulletin of the American Physical Society
APS March Meeting 2019
Volume 64, Number 2
Monday–Friday, March 4–8, 2019; Boston, Massachusetts
Session B64: Biopolymers I (DNA, RNA, Biocompatible, Gels)
11:15 AM–2:03 PM,
Monday, March 4, 2019
BCEC
Room: 259B
Sponsoring
Unit:
DBIO
Abstract: B64.00006 : Microfluidic delivery of cutting enzymes for fragmentation of surface-adsorbed DNA molecules.*
12:15 PM–12:27 PM
Presenter:
Julia Budassi
(Stony Brook University)
Authors:
Julia Budassi
(Stony Brook University)
Alan Gan
(East Brunswick High School, East Brunswick, NJ 08816)
Albert Tian
(Ward Melville High School, E. Setauket, NY 11733)
Jonathan Sokolov
(Stony Brook University)
methacrylate (PMMA) coated silicon substrate by ‘molecular combing’ [1]. Microfluidic channels were formed into polydimethylsiloxane (PDMS) stamps using the soft lithography technique. The channel widths varied from 3.5 to 7 microns. The channels were pre-filled with a buffer solution by the channel outgas method [2]. Bovine serum albumin was diffused into the channels to coat the PDMS surfaces (which would otherwise strongly adsorb the DNase I cutting enzyme). DNase I was allowed to diffuse into the channel for 1 hour, resulting in efficient and precise fragmentation of the DNAs over the full channel length of 10mm. Applications to DNA sequencing will be discussed.
[1] J. Monahan, A. Gewirth, R. Nuzzo, Anal. Chem. (2001) 73, 3193
[2] A. Bensimon et al, Science (1994) 277, 2096.
*Funding from NSF-AGEP (1344267) is gratefully ackowledged.
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