Bulletin of the American Physical Society
APS March Meeting 2018
Monday–Friday, March 5–9, 2018; Los Angeles, California
Session S49: Evolutionary Dynamics of Genomes I
11:15 AM–2:27 PM,
Thursday, March 8, 2018
LACC Room: 511A
Sponsoring Units: DBIO GSNP
Chair: Edo Kussell, New York University
Abstract: S49.00005 : Negative feedback as a facilitator of gene network evolution
12:27 PM–1:03 PM
Despite the examples of protein evolution via mutations in coding sequences, we have very limited understanding on gene network evolution via changes in cis-regulatory elements. Using the galactose network as a model, we studied how the regulatory promoters of the network contribute to the evolved network activity between two yeast species. In Saccharomyces cerevisiae, we combinatorially replaced all regulatory network promoters by their counterparts from Saccharomyces paradoxus, measured the resulting network inducibility profiles, and modeled the results. Lowering relative strength of GAL80-mediated negative feedback by replacing GAL80 promoter was necessary and sufficient to have high network inducibility levels as in S. paradoxus. This was achieved by increasing OFF-to-ON phenotypic switching rates. Competitions performed among strains with or without the GAL80 promoter replacement showed strong relationships between network inducibility and fitness. Our results support the hypothesis that gene network activity can evolve by optimizing the strength of negative-feedback regulation.
The American Physical Society (APS) is a non-profit membership organization working to advance the knowledge of physics.
1 Physics Ellipse, College Park, MD 20740-3844
Editorial Office 1 Research Road, Ridge, NY 11961-2701 (631) 591-4000
Office of Public Affairs 529 14th St NW, Suite 1050, Washington, D.C. 20045-2001 (202) 662-8700