Bulletin of the American Physical Society
APS March Meeting 2018
Monday–Friday, March 5–9, 2018; Los Angeles, California
Session L60: Poster Session II
Wednesday, March 7, 2018
LACC Room: West Hall A
Abstract: L60.00095 : Investigating pressure denaturation using the high pressure small angle x-ray scattering*
(Rensselaer Polytechnic Institute)
The continuous progress in the field of basic molecular biosciences has a persistent goal of establishing and utilizing the rational correlations amongst the molecular structure and function of biomolecules. As a fundamental thermodynamic parameter, pressure affects a process that leads to decrease in overall volume in a closed system, following the Le Chateliers’s principle. Exposure to a high-pressure (hp) environment causes proteins to unfold in a way that allows structural information to be accessed, not accessible otherwise. Pressure also affects the tertiary structure by collapsing the internal cavities unlike chemically or thermally induced denaturation. Small angle x-ray scattering (SAXS) provides direct information of the structure and hence the deviations in the volume of macromolecules. The presentation will elaborate upon the recent advances in the multidimensional hp sciences, specifically oriented towards the application of the hp-SAXS capabilities at the Cornell High Energy Synchrotron Source, which is complimentary in nature to the information obtained using NMR and fluorescence spectroscopies.
*Cornell High Energy Synchrotron Source (CHESS) is supported by the National Science Foundation under award DMR-1332208.
The American Physical Society (APS) is a non-profit membership organization working to advance the knowledge of physics.
1 Physics Ellipse, College Park, MD 20740-3844
Editorial Office 1 Research Road, Ridge, NY 11961-2701 (631) 591-4000
Office of Public Affairs 529 14th St NW, Suite 1050, Washington, D.C. 20045-2001 (202) 662-8700