Bulletin of the American Physical Society
APS March Meeting 2018
Monday–Friday, March 5–9, 2018; Los Angeles, California
Session L02: Self-assembly of Nanomaterials: Supramolecular Self-assembly I
11:15 AM–2:03 PM,
Wednesday, March 7, 2018
LACC Room: 150B
Sponsoring Unit: DCP
Chair: Nicholas Kotov, University of Michigan - Ann Arbor
Abstract: L02.00002 : Measurements of the Assembly of Individual HIV-1 Gag Protein Capsids Around Spherical Templates*
11:51 AM–12:03 PM
Despite the abundance of structural information about virus capsids, relatively little is known about how they assemble. The small sizes of the capsids and the wide range of timescales involved in the assembly process make it difficult to directly measure the assembly kinetics. We use a microscopy method based on interferometric scattering that addresses both of these challenges, allowing us to measure the the size of individual capsids as a function of time. We apply this method to study the self-assembly of the Gag protein of HIV-1 around a spherical template that is coated in nucleic acid. We find that a simple Langmuir adsorption model can describe the growth kinetics of each capsid. In addition, the assembly rate has a strong concentration dependence, and the binding of capsid proteins is reversible.
*This work was supported in part by the Harvard Materials Research Science and Engineering Center, the Center for Nanoscale Systems at Harvard, and the National Science Foundation Graduate Research Fellowship Program.
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