Session D11: Focus Session: Bacterial Biophysics II

2:30 PM–5:30 PM, Monday, March 3, 2014
Room: 203

Sponsoring Unit: DBIO
Chair: Gerard Wong, University of California, Los Angeles

Abstract ID: BAPS.2014.MAR.D11.5

Abstract: D11.00005 : Single Cell Response to Time-dependent Stimuli using a Microfluidic Bioreactor

3:42 PM–3:54 PM

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Authors:

  Eric M. Johnson-Chavarria
    (Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign)

  Utsav Agrawal
    (Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign)

  Melikhan Tanyeri
    (Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign)

  Thomas E. Kuhlman
    (Department of Physics, University of Illinois at Urbana-Champaign)

  Charles M. Schroeder
    (Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign)

Cellular adaptation is critical for survival under uncertain or dynamic environmental conditions. Recent studies have reported the ability of biological systems to implement low-pass filters to distinguish high frequency noise in environmental stimuli from lower frequency input signals, yet we still lack a complete understanding of this phenomenon. In this work, we report a microfluidic-based platform for single cell analysis that provides dynamic control over periodic, time-dependent culture media. Single cells are confined in free solution by the sole action of gentle fluid flow, thereby enabling non-perturbative trapping of cells for long time scales. In this way, our microfluidic-based technique provides the ability to control external stimuli with precise methods while observing non-adherent cells over long timescales. Using this approach, we observed intranucleoid diffusion of genetic repressor proteins released from a chromosomal binding array. Overall, this microfluidic approach provides a direct method for sustaining periodic environmental conditions, measuring growth rates, and detecting gene expression of single cells in free solution.

To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2014.MAR.D11.5