Bulletin of the American Physical Society
APS March Meeting 2011
Volume 56, Number 1
Monday–Friday, March 21–25, 2011; Dallas, Texas
Abstract: S1.00176 : Tethering Peptides to Functionalized Self-Assembled Monolayers on Gold Through Two Chemical Linkers Using the Huisgen Cycloaddition
Author:
A biocompatible platform has been made by tethering a helical
peptide to a
surface at two points. The presence of the peptide should be an
ideal
interface between inorganic substrates and proteins. The
artificially
synthetized alpha-helical peptide composed of alternating leucine
and lysine
residues, with two residues replaced with cyanophenylalanine to
react with
two neighboring surface-bound azide groups is linked to the
azide-terminated
self-assembled monolayer through a tetrazole made by a Husigen
Cycloaddition. Surface analysis is done with ellipsometry, infrared
spectroscopy and x-ray photoelectron spectroscopy. The
cycloaddition and
reaction conditions are supported by similar reactions of other
smaller
molecules like Methoxybenzonitrile and controls show no
physisorption under
our reaction conditions. Reaction yields from 80 to 98 percent
are reported
from the optimized reactions. The helical structure of the
peptide in
solution has been confirmed under our reaction conditions with
circular
dichroism and the peptide amide I and II modes studied by infrared
spectroscopy and their comparison with a computational model of
the peptide
showed that the peptide is probably randomly oriented on the surface.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2011.MAR.S1.176
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