Bulletin of the American Physical Society
APS March Meeting 2011
Volume 56, Number 1
Monday–Friday, March 21–25, 2011; Dallas, Texas
Abstract: S1.00149 : The translocation time of DNA and protein molecules in solid-state nanopores
Author:
The time that a biopolymer takes to translocate through a nanopore contains
the properties of the polymer including its size, conformation, electrical
charge and charge distribution. We measured the dependence of the
translocation times on the size, charge and charge distribution, voltage,
and conformation states of DNA and protein molecules. To quantitatively fit
the time distributions measured, 1-D Langevin and 1-D Fokker-Planck
equations were used for DNA and native state proteins. Kramers reaction rate
theory was used to fit the time distribution of unfolded proteins. It was
observed that native-state protein and DNA translocation approximately
follows simple one-dimensional biased diffusion of charged particles. Due to
the heterogeneous charge sequence of polypeptides, unfolded proteins obey a
coupled electrophoretic and thermally activated process that is sequence
specific. Deviations between models and experimental results as well as
future challenges for single molecule DNA and protein characterization using
solid-state nanopores will be discussed.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2011.MAR.S1.149
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