Session V43: Focus Session: Translocation Through Nanopores II

8:00 AM–11:00 AM, Thursday, March 24, 2011
Room: A306/307

Sponsoring Units: DPOLY DBP
Chair: Johan Dubbeldam, Delft University of Technology, Netherlands

Abstract ID: BAPS.2011.MAR.V43.3

Abstract: V43.00003 : Nanopore DNA translocation studies of tri-oligomer DNA with two hybridization segments

8:24 AM–8:36 AM

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Authors:

  Venkat Balagurusamy
    (Brown University)

  Paul Weinger
    (Brown University)

  Xinsheng Ling
    (Brown University)

We have earlier detected 12-base hybridizations in trimer DNA complexes formed by three single-stranded DNA oligomers hybridized at their ends sequentially, using nanopores of $\sim $ 10 nm diameter [1]. These complexes are connected to a polystyrene bead at one end to slow down their translocation. Here, we report translocation experiments at different voltages with nanopores $\sim $ 5 nm diameter. The measured time lapses between the passage of consecutive double-strand DNA segments in a trimer complex allow us to study the translocation dynamics. The measured mean-first-passage time between two consecutive hybridization segments is found to be consistent with theoretical estimates based on the Fokker-Planck equation.\\[4pt] [1] V.S.K.Balagurusamy, P.Weinger and X.S.Ling, \textit{Nanotechnology} \underline {21}, 335102 (2010).

To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2011.MAR.V43.3